SIMULTANEOUS MEASUREMENT OF RETICULOCYTE AND RED-BLOOD-CELL INDEXES IN HEALTHY-SUBJECTS AND PATIENTS WITH MICROCYTIC AND MACROCYTIC ANEMIA
SIMULTANEOUS MEASUREMENT OF RETICULOCYTE AND RED-BLOOD-CELL INDEXES IN HEALTHY-SUBJECTS AND PATIENTS WITH MICROCYTIC AND MACROCYTIC ANEMIA
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DOI：
10.1182/blood.v85.3.818.bloodjournal853818
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发表时间：
1995-02-01
期刊：
影响因子：
20.3
通讯作者：
MICCIULLI, G
中科院分区：
文献类型：
--
作者：
DONOFRIO, G;CHIRILLO, R;MICCIULLI, G
采用德国勒沃库森Sayer H*3血细胞分析仪，测定了健康人、缺铁性小红细胞增多症患者(58例)、杂合性β-地中海贫血患者(40例)和大细胞增多症患者(28例)的红细胞和网织红细胞指数。我们发现，在所有病例中，网织红细胞比成熟红细胞大24%到35%，而血红蛋白浓度低16%到25%，血红蛋白含量相似。在所有四组中，无论红细胞大小，红细胞和网织红细胞指数之间的相关性都非常密切(体积r=.928，血红蛋白浓度r=.929，血红蛋白含量r=.972)。网织红细胞与红细胞平均体积之比(MCV)始终在1以上，仅有4例患者出现MCV倒置。它总是突然和短暂的，并与红细胞生成变化有关，导致产生不同体积的红细胞(治疗巨幼细胞性贫血、功能性铁缺乏症、骨髓移植)。在两例骨髓移植中，网织红细胞的体积在条件化疗后的再生期下降，然后迅速增加，达到比以前更高的值；这种巨大网织红细胞的产生是植入的最早迹象。(C)1995年由美国血液病学会主办。
Using the new Sayer H*3 hematology analyzer (Leverkusen, Germany), we have determined red blood cell and reticulocyte indices in 64 healthy subjects, in patients with microcytosis due to iron deficiency (58 patients) and heterozygous beta-thalassemia (40 patients), and in patients with macrocytosis (28 patients). We found in all cases that reticulocytes were larger than mature red cells by 24% to 35%, with a hemoglobin concentration 16% to 25% lower and a similar hemoglobin content. The correlation between red cell and reticulocyte indices was strikingly tight (r = .928 for volume, r = .929 for hemoglobin concentration, r = .972 for hemoglobin content) in all four groups, regardless of red blood cell size. The ratio of reticulocyte to red blood cell mean corpuscolar volume (MCV ratio) was constantly above 1. Inversion of the MCV ratio was observed only in four patients. It was always abrupt and transitory and was associated with erythropoietic changes leading to the production of red blood cells of a different volume (treatment of megaloblastic anemia, functional iron deficiency, bone marrow transplantation). In two cases of marrow transplantation, reticulocyte volume fell during the aplastic phase after conditioning chemotherapy and then rapidly increased up to values higher than before; this production of macroreticulocytes was the earliest sign of engraftment. (C) 1995 by The American Society of Hematology.