Over-expression of microRNA169 confers enhanced drought tolerance to tomato
Over-expression of microRNA169 confers enhanced drought tolerance to tomato
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DOI：
10.1007/s10529-010-0436-0
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
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作者：
Zhang, Xiaohui;Zou, Zhe;Ye, Zhibiao
植物miRNA调控多种发育和生理过程，包括干旱响应。我们发现干旱胁迫诱导了Sly-miR 169在番茄（Solanum lycopersicum）中的积累。因此，Sly-miR 169靶基因，即三个核因子Y亚基基因（SlNF-YA 1/2/3）和一个多药耐药相关蛋白基因（SlMRP 1），在干旱胁迫下显著下调。miR 169家族成员Sly-miR 169 c在番茄中的组成型过表达可有效下调靶基因的转录。与非转基因植株相比，过表达Sly-miR 169 c的转基因植株气孔开度降低，蒸腾速率降低，叶片失水减少，耐旱性增强。我们的研究是第一个提供证据表明，Sly-miR 169 c负调控番茄干旱响应气孔运动。
Plant miRNA regulates multiple developmental and physiological processes, including drought responses. We found that the accumulation of Sly-miR169 in tomato (Solanum lycopersicum) was induced by drought stress. Consequently, Sly-miR169 targets, namely, three nuclear factor Y subunit genes (SlNF-YA1/2/3) and one multidrug resistance-associated protein gene (SlMRP1), were significantly down-regulated by drought stress. Constitutive over-expression of a miR169 family member, Sly-miR169c, in tomato plant can efficiently down-regulate the transcripts of the target genes. Compared with non-transgenic plants, transgenic plants over-expressing Sly-miR169c displayed reduced stomatal opening, decreased transpiration rate, lowered leaf water loss, and enhanced drought tolerance. Our study is the first to provide evidence that the Sly-miR169c negatively regulates stomatal movement in tomato drought responses.