MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3
MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3
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MiR-212-3p 通过靶向 SGK3 抑制胶质母细胞瘤细胞增殖
DOI：
10.1007/s11060-015-1736-y
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发表时间：
2015-05-01
影响因子：
3.9
通讯作者：
Zhao, Shiguang
中科院分区：
文献类型：
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作者：
Liu, Huailei;Li, Chenguang;Zhao, Shiguang
多形性胶质母细胞瘤（GBM）是人类最恶性的脑肿瘤。已有研究表明，microRNA在GBM细胞的发育和增殖中起着重要作用。在这里，我们定义了miR-212-3p调节GBM增殖的机制。在本研究中，我们发现miR-212-3p的表达在GBM中显著下调，并与血清和糖皮质激素诱导激酶3 （SGK3）呈负相关。过表达miR-212-3p或SGK3沉默均可降低GBM细胞的活力。此外，miR-212-3p直接结合SGK3的3'UTR，抑制其mRNA和蛋白的表达。SGK3的过表达挽救了miR-212-3p诱导的GBM细胞增殖下降。重要的是，miR-212-3p在体内也抑制肿瘤生长。总之，我们的研究结果表明，miR-212-3p通过直接靶向SGK3抑制GBM细胞的增殖，并且可能作为GBM的新治疗靶点。
Glioblastoma multiforme (GBM) is the most malignant brain tumor in humans. Previous studies have demonstrated that microRNA plays important roles in the development and proliferation of GBM cells. Here we defined the mechanism by which miR-212-3p regulated the proliferation of GBM. In this study, we showed that miR-212-3p expression was significantly down-regulated and negatively correlated with serum and glucocorticoid-inducible kinase 3 (SGK3) in GBM. Either over-expression of miR-212-3p or silence of SGK3 decreased viability of GBM cells. Moreover, miR-212-3p directly bound to 3′UTR of SGK3 and inhibited its mRNA and protein expression. And over-expression of SGK3 rescued the decreased proliferation of GBM cells induced by miR-212-3p. Importantly, miR-212-3p also suppressed tumor growth in vivo. Collectively, our results demonstrated that miR-212-3p inhibited proliferation of GBM cells by directly targeting SGK3, and could potentially serve as a new therapeutic target for GBM.