Transcriptomic dynamics provide an insight into the mechanism for silicon mediated alleviation of salt stress in cucumber plants
Transcriptomic dynamics provide an insight into the mechanism for silicon mediated alleviation of salt stress in cucumber plants
复制标题
转录组动力学提供了对硅介导的黄瓜植物盐胁迫缓解机制的深入了解
DOI：
10.1016/j.ecoenv.2019.02.075
复制
发表时间：
2019
影响因子：
6.8
通讯作者：
Gong Haijun
中科院分区：
文献类型：
--
作者：
Zhu Yongxing;Yin Junliang;Liang Yufei;Liu Jiaqi;Jia Jianhua;Huo Heqiang;Wu Zefeng;Yang Ruolin;Gong Haijun
盐分降低了农作物的产量和质量。硅对盐胁迫下植物的生长发育具有重要的促进作用。然而，人们对这一机制仍然知之甚少。为了鉴定可能被精心安排以提高黄瓜植物的耐盐性的基因或基因网络，我们在添加Si的存在或不存在下对对照和盐胁迫黄瓜叶片的转录组进行了测序。黄瓜“津优1号”幼苗在不加或加0.3mM硅的情况下经受盐胁迫（75mM NaCl）。植物生长，光合气体交换和转录动力学进行了研究。结果表明，施硅能提高盐胁迫下黄瓜幼苗的生长和光合性能。比较转录组分析表明，硅对黄瓜转录组的形成起着重要作用：对照条件下，硅处理改变了1469个基因的表达，这些基因主要参与离子转运、激素和信号转导、生物合成和代谢过程以及胁迫和防御反应。在盐胁迫下，1482个与代谢过程和对环境刺激的反应相关的推定功能基因的表达水平发生了变化。Si处理将盐胁迫黄瓜的转录组移回到对照的转录组，证明在盐胁迫下上调或下调的708和774个基因中，绝大多数（分别为609和595）恢复到正常表达水平。这些结果表明，硅可能作为一种激发子预处理黄瓜植株，诱导耐盐性。该研究有助于理解硅介导的耐盐机制，为盐碱地作物生产中硅的应用提供理论依据。
Salinity decreases the yield and quality of crops. Silicon (Si) has been widely reported to have beneficial effects on plant growth and development under salt stress. However, the mechanism is still poorly understood. In an attempt to identify genes or gene networks that may be orchestrated to improve salt tolerance of cucumber plants, we sequenced the transcriptomes of both control and salt-stressed cucumber leaves in the presence or absence of added Si. Seedlings of cucumber ‘JinYou 1’ were subjected to salt stress (75 mM NaCl) without or with addition of 0.3 mM Si. Plant growth, photosynthetic gas exchange and transcriptomic dynamics were investigated. The results showed that Si addition improved the growth and photosynthetic performance of cucumber seedlings under salt stress. The comparative transcriptome analysis revealed that Si played an important role in shaping the transcriptome of cucumber: the expressions of 1469 genes were altered in response to Si treatment in the control conditions, and these genes were mainly involved in ion transport, hormone and signal transduction, biosynthetic and metabolic processes, and stress and defense responses. Under salt stress alone, 1482 genes with putative functions associated with metabolic processes and responses to environmental stimuli have changed their expression levels. Si treatment shifted the transcriptome of salt-stressed cucumber back to that of the control, as evidenced that among the 708 and 774 genes that were up- or down-regulated under salt stress, a large majority of them (609 and 595, respectively) were reverted to the normal expression levels. These results suggest that Si may act as an elicitor to precondition cucumber plants and induce salt tolerance. The study may help us understand the mechanism for silicon-mediated salt tolerance and provide a theoretical basis for silicon application in crop production in saline soils.