Effects of age, vitamin D3, and fructooligosaccharides on bone growth and skeletal integrity of broiler chicks
Effects of age, vitamin D3, and fructooligosaccharides on bone growth and skeletal integrity of broiler chicks
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DOI：
10.3382/ps.2011-01475
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发表时间：
2011-11-01
期刊：
影响因子：
4.4
通讯作者：
Ricke, S. C.
中科院分区：
文献类型：
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作者：
Kim, W. K.;Bloomfield, S. A.;Ricke, S. C.
采用外周定量CT和力学测试方法，研究了日龄、维生素D-3和低聚果糖（FOS）对肉仔鸡骨密度（MID）、骨矿含量（BMC）、骨皮质厚度、骨皮质和骨小梁面积以及力学性能的影响。试验选用54只1日龄雄性肉仔鸡，采用半封闭式育雏器，饲喂玉米-大豆混合型日粮7 d。7 d后。将小鸡随机分配到围栏中，每个围栏3只鸡。每个处理重复3次。共有6种治疗：1）早期对照（对照1）; 2）对照2; 3）125 μ g/kg维生素D-3; 4）250 μ g/kg维生素D-3; 5）2%FOS;和6）4%FOS。对照组1鸡饲喂对照肉鸡饮食，并在第14天处死以收集股骨用于骨分析。其余各组均于第21天处死。3周龄雏鸡股骨中段皮质骨密度、骨矿物质含量、骨面积、骨厚度和骨髓面积均大于2周龄雏鸡（P值分别为0.016、0.0003、0.0002、0.01和0.0001）。鸡股骨近端的总骨密度、皮质骨密度和骨小梁骨密度不受年龄的影响。然而，BMC和骨面积受年龄影响显著。2周龄雏鸡股骨的刚度和极限载荷显著低于3周龄雏鸡（P = 0.0001），而极限应力和弹性模量显著高于3周龄雏鸡（P = 0.0001）。饲喂250 μ g/kg维生素D-3的雏鸡表现出显著更大的中段皮质BMC（P = 0.04），骨面积（P = 0.04）。和厚度（P = 0.03）显著高于对照组2、2%FOS和4%FOS。总之，我们的研究表明，高水平的维生素D-3可以增加肉鸡的骨骼生长和矿物质沉积。然而. FOS对骨生长和骨骼完整性没有任何有益的影响。年龄是影响肉仔鸡骨骼完整性和力学性能的重要因素
A study was conducted to evaluate the effects of age, vitamin D-3, and fructooligosaccharides (FOS) on bone mineral density (MID), bone mineral content (BMC), cortical thickness, cortical and trabecular area, and mechanical properties in broiler chicks using peripheral quantitative computed tomography and mechanical testing. A total of 54 male broiler chicks (1 d old) were placed in battery brooders and fed a corn-soybean starter diet for 7 d. After 7 d. the chicks were randomly assigned to pens of 3 birds each. Each treatment was replicated 3 times. There were 6 treatments: 1) early age control (control 1); 2) control 2; 3) 125 mu g/kg of vitamin D-3; 4) 250 mu g/kg of vitamin D-3; 5) 2% FOS); and 6) 4% FOS. The control 1 chicks were fed a control broiler diet and killed on d 14 to collect femurs for bone analyses. The remaining groups were killed on d 21. Femurs from 3-wk-old chicks showed greater midshaft cortical BMD, BMC, bone area, thickness, and marrow area than those from 2-wk-old chicks (P = 0.016, 0.0003, 0.0002, 0.01, and 0.0001, respectively). Total, cortical, and trabecular BMD of chick proximal femurs were not influenced by age. However, BMC and bone area were significantly affected by age. The femurs of 2-wk-old chicks exhibited significantly lower stiffness and ultimate load than those of 3-wk-old chicks (P = 0.0001), whereas ultimate stress and elastic modulus of the femurs of 2-wk-old chicks were significantly higher than that of femurs of 3-wk-old chicks (P = 0.0001). Chicks fed 250 mu g/kg of vitamin D-3 exhibited significantly greater midshaft cortical BMC (P = 0.04), bone area (P = 0.04). and thickness (P = 0.03) than control 2, 2% FOS, or 4% FOS chicks. In summary, our study suggests that high levels of vitamin D-3 can increase bone growth and mineral deposition in broiler chicks. However. FOS did not have any beneficial effects on bone growth and skeletal integrity. Age is an important factor influencing skeletal integrity and mechanical properties in broiler chicks