Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles
Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles
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DOI：
10.4012/dmj.2019-176
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发表时间：
2021-02-01
影响因子：
2.5
通讯作者：
Akasaka, Tsukasa
中科院分区：
文献类型：
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作者：
Nishida, Erika;Miyaji, Hirofumi;Akasaka, Tsukasa
氟化锌玻璃纳米颗粒（Zinc-F）通过酸碱反应释放出几种离子，如氟离子、锌离子和钙离子。本研究的目的是评价锌-F的抗菌和细胞毒性特性。抗菌试验表明，锌-F洗脱液显着降低浊度和菌落形成单位的变形链球菌和内氏放线菌，相比，不含锌离子的氟铝硅酸钙玻璃纳米粒子。在活/死染色中，与对照（未施用）相比，锌-F显著减少了绿色染色的细菌细胞，表明活细胞。锌-F涂层的人牙本质对S. mutans和A.内氏菌生物膜形成。此外，锌-F在成骨细胞和成纤维细胞中显示出低细胞毒性作用。因此，我们的研究结果表明，锌-F通过多离子释放表现出抗菌和生物相容性。
Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium ions, through acid-base reactions. The aim of this study was to evaluate the antibacterial and cytotoxic properties of Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced the turbidity and colony-forming units of Streptococcus mutans and Actinomyces naeslundii, compared to that of calcium-fluoroaluminosilicate glass nanoparticles without zinc ions. In live/dead staining, Zinc-F eluate significantly decreased green-stained bacterial cells, indicating live cells, compared with the control (no application). Human dentin coated with Zinc-F showed suppressed S. mutans and A. naeslundii biofilm formation. Additionally, Zinc-F eluate showed low cytotoxic effects in osteoblastic and fibroblastic cells. Therefore, our findings suggested that Zinc-F exhibits antibacterial and biocompatible properties through multiple-ion release.