Oral Bacteria as Potential Probiotics for the Pharyngeal Mucosa
Oral Bacteria as Potential Probiotics for the Pharyngeal Mucosa
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DOI：
10.1128/aem.00109-10
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发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Mora, Diego
中科院分区：
文献类型：
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作者：
Guglielmetti, Simone;Taverniti, Valentina;Mora, Diego
本文所述的研究旨在选择口腔细菌，这些细菌显示出与它们作为咽粘膜益生菌的潜在用途相兼容的特性。我们在研究中纳入了从健康供体的咽部新分离的56种细菌，这些细菌在种内水平上被鉴定，并在体外对其益生菌潜力进行了表征。通过实验，我们选择了两种潜在的益生菌菌株（唾液链球菌RS1和ST3），并与原型口腔益生菌唾液链球菌K12进行比较。这3株菌株均能有效地与FaDu人咽上皮细胞结合，从而拮抗化脓性链球菌的粘附和生长。所有患者均对常规用于控制上呼吸道感染的多种抗生素敏感。FaDu细胞对RS1、ST3和K12有不同的免疫应答。RS1和ST3在基线和白细胞介素-1 β (IL-1 β)诱导后调节NF-kappa B的激活和偏倚的促炎细胞因子。总之，我们认为所选的共生链球菌代表了潜在的咽部益生菌候选者。它们对宿主具有良好的适应性，并具有潜在的免疫调节和抗炎特性。
The research described here was aimed at the selection of oral bacteria that displayed properties compatible with their potential use as probiotics for the pharyngeal mucosa. We included in the study 56 bacteria newly isolated from the pharynges of healthy donors, which were identified at the intraspecies level and characterized in vitro for their probiotic potential. The experiments led us to select two potential probiotic bacterial strains (Streptococcus salivarius RS1 and ST3) and to compare them with the prototype oral probiotic S. salivarius strain K12. All three strains efficiently bound to FaDu human epithelial pharyngeal cells and thereby antagonized Streptococcus pyogenes adhesion and growth. All were sensitive to a variety of antibiotics routinely used for the control of upper respiratory tract infections. Immunological in vitro testing on a FaDu layer revealed different responses to RS1, ST3, and K12. RS1 and ST3 modulated NF-kappa B activation and biased proinflammatory cytokines at baseline and after interleukin-1 beta (IL-1 beta) induction. In conclusion, we suggest that the selected commensal streptococci represent potential pharyngeal probiotic candidates. They could display a good degree of adaptation to the host and possess potential immunomodulatory and anti-inflammatory properties.