Natural Killer Cells as Biomarkers of Hyperbaric Stress During a Dry Heliox Saturation Dive
Natural Killer Cells as Biomarkers of Hyperbaric Stress During a Dry Heliox Saturation Dive
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DOI：
10.3357/asem.2528.2010
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发表时间：
2010-05-01
影响因子：
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通讯作者：
Hokland, Marianne E.
中科院分区：
文献类型：
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作者：
Krog, Jan;Tonnesen, Else K.;Hokland, Marianne E.
KROG J，TONNESEN EK，JEPSEN CF，PARNER SEGADAL K，HOPE A，ULVIK RJ，HOKLAND ME. Natural killer cells as biomarkers of hyperbasic stress during a dry heliov saturation dive. Aviat Space Environ Med 2010; 81：467- 74简介：潜水、高压氧和减压被描述为人体免疫系统各种组分改变的诱导物，例如循环淋巴细胞的分布。假设在高压暴露期间监测特定淋巴细胞亚群，包括T细胞和NK细胞亚群，可以作为高压应激的生物标志物。八名经验丰富的饱和潜水员和八名未进行过深度饱和潜水的参考受试者，在干氦氧饱和潜水至264 MPa（254 msw）的19个3天中，在潜水前和潜水过程中的不同时间点分离外周血单个核细胞，在4小时的Cr-51-γ-CD中评估NK细胞的细胞毒性。使用NK细胞敏感性肿瘤细胞系K562作为靶细胞的释放测定主要淋巴细胞亚群，特别强调NK细胞亚群，通过使用四色流式细胞术进行表型描绘。尽管在加压阶段潜水员和保持在舱外常氧条件下的参考受试者的NK细胞毒性显著增加，但潜水员的NK细胞毒性显著更高。这一发现，由于在高压期间先天性细胞免疫系统的特定部分可能被激活，暴露，表明监测特异性免疫功能可以作为高压诱导的炎症应激的生物标志物
KROG J, TONNESEN EK, JEPSEN CF, PARNER SEGADAL K, HOPE A, ULVIK RJ, HOKLAND ME. Natural killer cells as biomarkers of hyperbasic stress during a dry heliov saturation dive. Aviat Space Environ Med 2010; 81:467-74Introduction: Diving, hyperbaric oxygen, and decompression have been described as inducers of alterations in various components of the human immune system, such as the distribution of circulating lymphocytes Hypothetically, the monitoring of specific lymphocyte subsets during hyperbaric exposure, including T- and NK-cell subsets, can serve as biomarkers of hyperbaric stress Methods: Eight experienced saturation divers and eight reference subjects, naive to deep saturation diving, were examined Peripheral blood mononuclear cells were isolated before and at different points during a 19 3-d dry heliox saturation dive to 2 64 MPa (254 msw) The NK cell cytotoxicity was estimated in a 4-h Cr-51-release assay using the NK cell sensitive tumor cell-line K562 as target cells The major lymphocyte subpopulations, with special emphasis on the NK cell subsets, were phenotypically delineated by the use of 4-color flow cytometry Results. Although NK cell cytotoxicity increased significantly in the divers during the compression phase and the reference subjects who remained in normoxic conditions outside the chamber, the NK cell cytotoxicity was significantly higher in the divers Discussion: This finding, together with augmentation in the absolute number of circulating NK cells in the divers due to a possible activation of specific parts of the innate cellular immune system during hyperbaric exposure, suggests the monitoring of specific immune functions can be useful as biomarkers of hyperbaric-induced inflammatory stress