Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning.
Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning.
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通过表面微图案揭示微尺度结构在免疫突触功能中的作用。
DOI：
10.1007/978-1-4939-6881-7_17
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kam,LanceC
中科院分区：
文献类型：
--
作者：
Lee,Joung-Hyun;Kam,LanceC
The immune synapse has emerged as a compelling example of structural complexity within cell-cell interfaces. This chapter focuses on the use of microcontact printing to isolate and investigate how spatial organization of signaling molecules drives the function of immune cells. In the process detailed here, multiple rounds of microcontact printing are combined to create patterned surfaces that control the relative spatial localization of CD3 and CD28 signaling in T cells, effectively replacing an antigen presenting cell with an engineered surface. A set of approaches used to address key issues of T cell activation are described and discussed.
