Proton-detected nitrogen-14 NMR by recoupling of heteronuclear dipolar interactions using symmetry-based sequences
Proton-detected nitrogen-14 NMR by recoupling of heteronuclear dipolar interactions using symmetry-based sequences
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DOI：
10.1016/j.cplett.2007.07.060
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发表时间：
2007-09-04
影响因子：
2.8
通讯作者：
Bodenhausen, Geoffrey
中科院分区：
文献类型：
--
作者：
Cavadini, Simone;Abraham, Amiji;Bodenhausen, Geoffrey
N-14 和 H-1 之间的异核偶极相互作用与 R20(5)(9) 类型的基于对称的 RN 序列应用于以魔角旋转的样品中的质子，允许人们激发 N-14 单量子 (SQ) 或双量子 (DQ) 相干性，并将它们重新转换为可观察的质子相干性。这提供了通过旋转共振重新耦合异核偶极相互作用的替代方案。 SQ 和 DQ 光谱的双向相干传递过程的实验效率约为 9% 和 4%。质子的光谱是在 400 和 800 MHz 下获得的。 N-14 四极张量的参数可以在肽中确定，包括质子化胺中的旋转 (NH3+)-N-14-H-1 基团和酰胺基团中的刚性 (NH)-N-14-H-1 对。 (C) 2007 Elsevier B.V. 保留所有权利。
The recoupling of heteronuclear dipolar interactions between N-14 and H-1 with symmetry-based RN sequences of the R20(5)(9) type applied to protons in samples spinning at the magic angle allows one to excite N-14 single-quantum (SQ) or double-quantum (DQ) coherences and reconvert them into observable proton coherences. This offers an alternative to the recoupling of heteronuclear dipolar interactions by rotary resonance. The experimental efficiency of the two-way coherence transfer process is on the order of 9 and 4% for SQ and DQ spectroscopy. Spectra were obtained at 400 and 800 MHz for protons. The parameters of the N-14 quadrupolar tensors can be determined in peptides, both in rotating (NH3+)-N-14-H-1 groups in protonated amines and in rigid (NH)-N-14-H-1 pairs in amide groups. (C) 2007 Elsevier B.V. All rights reserved.