IMMUNOHISTOCHEMICAL LOCALIZATION OF CALCIUM-BINDING PROTEIN IN THE CEREBELLUM, HIPPOCAMPAL-FORMATION AND OLFACTORY-BULB OF THE RAT
IMMUNOHISTOCHEMICAL LOCALIZATION OF CALCIUM-BINDING PROTEIN IN THE CEREBELLUM, HIPPOCAMPAL-FORMATION AND OLFACTORY-BULB OF THE RAT
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DOI：
10.1016/0006-8993(82)90804-6
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
MILLER, JJ
中科院分区：
文献类型：
--
作者：
BAIMBRIDGE, KG;MILLER, JJ
用亲和层析纯化的兔抗人或羊抗鸡抗血清，对大鼠小脑、海马结构和嗅球中钙结合蛋白（CaBP）进行免疫组织化学定位。CaBP样免疫反应见于小脑浦肯野细胞、海马放射层齿状颗粒细胞、CA1区锥体细胞和散在中间神经元的胞体和树突以及嗅球球周围细胞。在这些结构中的每一个中的某些主要细胞类型中，包括小脑颗粒细胞、CA3区域中的海马锥体细胞以及嗅球中的僧帽细胞和颗粒细胞中，染色明显不存在。还观察到小脑中的免疫反应纤维，推测对应于来自下橄榄核的攀爬纤维输入和向小脑深层核的传出投射。在海马中，强烈的染色存在于苔藓纤维投射到CA3区和内嗅皮质的穿通投射的末端区域。
The immunohistochemical localization of Ca-binding protein (CaBP) in the cerebellum, hippocampal formation and olfactory bulb of the rat was examined using rabbit anti-human or sheep anti-chick antisera purified by affinity chromatography. CaBP-like immunoreactivity was observed within the somata and dendrites of cerebellar Purkinje cells, dentate granule cells, CA1 pyramidal cells and scattered interneurons in the stratum radiatum of the hippocampus, and periglomerular cells in the olfactory bulb. Staining was conspicuously absent in certain major cell types in each of these structures including cerebellular granule cells, hippocampal pyramidal cells in the CA3 region and both mitral and granule cells in the olfactory bulb. Immunoreactive fibers in the cerebellum presumably corresponding to climbing fiber inputs from the inferior olive and efferent projections to the deep cerebellar nuclei, were also observed. In the hippocampus, intense staining was present in the mossy fiber projection to the CA3 region and in the terminal regions of the perforant projection from entorhinal cortex.