Great promoting effect of high irradiance from germination on flowering in Arabidopsis thaliana — a process of photo-acclimation
Great promoting effect of high irradiance from germination on flowering in Arabidopsis thaliana — a process of photo-acclimation
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DOI：
10.1007/s11099-007-0042-0
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发表时间：
2007-06
期刊：
影响因子：
2.7
通讯作者：
S. Moharekar;R. Tanaka;K. Ogawa;A. Tanaka;Toshihiko Hara
中科院分区：
文献类型：
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作者：
S. Moharekar;R. Tanaka;K. Ogawa;A. Tanaka;Toshihiko Hara
拟南芥，ch 1 -1（无叶绿素突变体）、gi-1（GI缺陷突变体）、cry 2 -1（蓝光光感受器β 2缺陷突变体）和哥伦比亚（Col;野生型）从萌发开始在宽范围的辐照度（I）下生长，并研究Ion对存活、发育和开花的影响。在低和中等I（<300 µmol m−2s−1）下，开花时间和开花时的植株大小表现出很大的差异，而在高I（>500 µmol m−2s−1）下，这些特征几乎相同。因此，在高I条件下，ch 1 -1、gi-1、cry 2 -1和Col的发育和开花几乎趋于一致。开花时间与I呈负相关，在A.拟南芥的生长与叶绿素（Chl）含量的减少和叶黄素循环库和膜结合APX活性的增加有关（EC 1.11.1.11），这表明氧化胁迫的增加诱导提早开花。gi-1和cry 2 - 1的植株在1 000 µmol m−2s−1下存活，而Col和ch 1 - 1则死亡，表明GIorb 2缺失突变体比Col和Chlb缺失突变体更耐光胁迫。因此，高I促进了拟南芥从萌发到开花的发育和开花时间，涉及光合机构的调节，这种促进作用不依赖于开花诱导基因GIOR 2的功能。这可以看作是A.拟南芥在高温下的生存和繁殖能力。
Arabidopsis thalianaL.,ch1-1(chlorophyllb-less mutant),gi-1(GIdeficient mutant),cry2-1(blue-light-photoreceptorCRY2deficient mutant), and Columbia (Col; wild ecotype) were grown under broad range of irradiances (I) from the beginning of germination and the effect ofIon the survival, development, and flowering was studied. Under low and moderateI(<300 µmol m−2s−1), flowering time and plant size at flowering showed great variations amongch1-1, gi-1, cry2-1, and Col, whereas under higherI(>500 µmol m−2s−1), these characteristics were almost the same. Hence under highI, development and flowering ofch1-1, gi-1, cry2-1, and Col converged to almost the same state. Flowering time was negatively correlated withI, and under highIacclimation inA. thalianawas associated with a decrease in chlorophyll (Chl) content and increases in xanthophyll cycle pool and membrane-bound APX activity (EC 1.11.1.11) suggesting that an increase in oxidative stress induces earlier flowering. The plants ofgi-1andcry2-1survived but Col andch1-1died under 1 000 µmol m−2s−1, showing that mutants deficient inGIorCRY2are more photo-stress-tolerant than Col and the Chlb-less mutant. Hence highIpromotes in plants ofArabidopsisraised from germination till flowering the development and flowering time involving modulation of the photosynthetic apparatus, and this promoting effect is independent of the functions of flower-inducingGIorCRY2gene. This can be regarded as photo-acclimation ofA. thalianafor survival and reproduction under highI.