Can quantitative RT-PCR for SARS-CoV-2 help in better management of patients and control of coronavirus disease 2019 pandemic.
Can quantitative RT-PCR for SARS-CoV-2 help in better management of patients and control of coronavirus disease 2019 pandemic.
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可以定量用于SARS-COV-2的RT-PCR有助于更好地治疗患者并控制冠状病毒病2019年大流行。
DOI：
10.4103/ijmm.ijmm_20_380
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发表时间：
2020-07
影响因子：
1.6
通讯作者：
Ahmad H
中科院分区：
文献类型：
--
作者：
Rattan A;Ahmad H
2019年冠状病毒病(新冠肺炎)的病原体SARS-CoV-2的出现，代表着一场前所未有的突发公共卫生事件。全球遏制工作的重点是检测、追踪接触者并对那些发现新冠肺炎阳性的人进行治疗(隔离)。由于这种新型RNA病毒的一些毒株的全基因组序列在2020年1月初已经公开，因此设计了一些实时聚合酶链式反应(RT-PCR)方法并用于诊断这种感染。大多数RT-PCR用于新冠肺炎的定性报告(无论是否检测到SARS-CoV-2)，但已被用于基于周期阈值的病毒载量的半定量估计。我们的手稿讨论了定量聚合酶链式反应检测在新冠肺炎中的应用及其对患者管理的好处。
The emergence of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), represents a public health emergency of unprecedented proportion. The global containment efforts have been focused on testing, tracing of contacts and treatment (isolation) of those found COVID-19 positive. Since the whole genome sequences of a number of strains of this novel RNA virus were available in the public domain by early January 2020, a number of real-time polymerase chain reaction (RT-PCR) protocols were designed and used for diagnosis of this infection. Most RT-PCRs are designed for qualitative COVID-19 reporting (SARS-CoV-2 detected or not detected), but have been used for semi-quantitative estimation of viral load based on cycle threshold value. Our manuscript discusses the utility of quantitative PCR testing for COVID-19 and its patient management benefits.