Structural relationships in the adenylate kinase family.
Structural relationships in the adenylate kinase family.
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DOI：
10.1111/j.1432-1033.1986.tb10132.x
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发表时间：
1986-11
期刊：
影响因子：
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通讯作者：
G. Schulz;E. Schiltz;A. Tomasselli;R. Frank;M. Brune;A. Wittinghofer;R. Schirmer
中科院分区：
文献类型：
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作者：
G. Schulz;E. Schiltz;A. Tomasselli;R. Frank;M. Brune;A. Wittinghofer;R. Schirmer
五个远亲腺苷酸激酶的序列已经对齐。根据已知的猪肌肉胞质同工酶1 （AK1）的三维结构，讨论了氨基酸的局部守恒。相似性曲线清晰地勾勒出AK1空间结构间隙中的活性位点。比对结果进一步表明，根据链中间约30个残基的特定片段的存在与否，酶家族可以被细分为小变体和大变体。大变异的额外片段是强保守的。
The sequences of five distantly related adenylate kinases have been aligned. The local conservation of amino acids is discussed in the light of the known three-dimensional structure of one of the enzymes, the cytosolic isoenzyme 1 (AK1) from porcine muscle. The similarity profile outlines clearly the active site in the cleft of the spatial structure of AK1. The alignment reveals further that the enzyme family can be subdivided into small and large variants according to the presence or absence of a particular segment of about 30 residues in the middle of the chain. The extra segments of the large variants are strongly conserved.