Electrical impedance myography detects age-related muscle change in mice
Electrical impedance myography detects age-related muscle change in mice
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DOI：
10.1371/journal.pone.0185614
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发表时间：
2017-10-19
期刊：
影响因子：
3.7
通讯作者：
Rutkove, Seward B.
中科院分区：
文献类型：
--
作者：
Arnold, W. David;Taylor, Rebecca S.;Rutkove, Seward B.
肌肉质量和力量的丧失是导致老年人功能受损的最重要因素之一。需要方便和非侵入性的生物标志物，可以容易地识别和跟踪与年龄相关的肌肉变化。先前的数据表明，电阻抗肌图(EIM)有潜力发挥这一作用。在这项研究中，我们调查了EIM与其他标准肌肉结构和功能指标相比在老年小鼠和年轻小鼠中的变化。19只约25个月龄的雄性小鼠和19只3个月龄的雄性小鼠使用标准方法对右侧腓肠肌进行表面多频EIM。测定前后肢握力、坐骨神经复合肌肉动作电位波幅和腓肠肌原位作用力；处死后用标准组织学方法评价腓肠肌纤维大小。计算Spearman相关系数以研究EIM与肌肉特征之间的关系。老年动物EIM显示50 kHz阻抗相显著降低(p
Loss of muscle mass and strength represents one of the most significant contributors to impaired function in older adults. Convenient and non-invasive biomarkers are needed that can readily identify and track age-related muscle change. Previous data has suggested electrical impedance myography (EIM) has the potential to serve in this capacity. In this study we investigated how changes in EIM compared with other standard measures of muscle structure and function in aged compared with young mice. A total of 19 male mice aged approximately 25 months and 19 male mice aged 3 months underwent surface multifrequency EIM of the right gastrocnemius muscle using standard methods. Fore and hind limb grip strength, sciatic compound muscle action potential amplitude, and in-situ force of the gastrocnemius were also measured; after sacrifice, gastrocnemius myofiber size was assessed using standard histology. Spearman correlation coefficients were calculated to investigate the association between EIM and muscle characteristics. EIM in aged animals demonstrated significantly lower 50 kHz impedance phase (p