Phenotypic identification of the subgroups of murine T-cell receptor alphabeta+ CD4+ CD8- thymocytes and its implication in the late stage of thymocyte development.
Phenotypic identification of the subgroups of murine T-cell receptor alphabeta+ CD4+ CD8- thymocytes and its implication in the late stage of thymocyte development.
复制标题
鼠 T 细胞受体 Alphaa CD4 CD8- 胸腺细胞亚群的表型鉴定及其在胸腺细胞发育后期的意义。
作者：
Q. Ge;W. F. Chen
髓质型 CD4+ CD8- [CD4 单阳性 (SP)] 胸腺细胞的表型分析揭示了该细胞群内的表型异质性。成熟外周T细胞的特征表型可以唯一标记为Qa-2+ HSA- CD69-，而在髓质型CD4 SP胸腺细胞中，许多标记物的表达模式有很大不同。这表明群体中存在许多亚群，这反映出髓质型CD4 SP胸腺细胞可能经历表型成熟。根据双色流式细胞术的结果，通过 Qa-2、HSA、CD69、3G11 和 6C10 分子的表达能力鉴定了七种离散表型。因此，表型前体-后代关系可设想为： 3G11- 6C10+ CD69+ HSAhi -->3G11+ 6C10+ CD69+ HSAhi --> 3G11+ 6C10- CD69+ HSAint -->3G11+ 6C10- CD69- HSAint Qa-2- -->3G11+ HSA-/lo Qa-2lo。在3G11+6C10-CD69-HSAint Qa-2-阶段，可以启动分支途径，产生3G11-HSA1度Qa-2-细胞，然后，其依次发育成3G11-HSA-/loQa-2hi细胞，这是最成熟的CD4 SP细胞的次要亚群。与这一预测途径一致，实验表明前两个亚组仍然对可的松敏感，而其他亚组则对可的松具有抗性。最后两个 Qa-2 阳性亚群中的细胞可能已准备好迁移到外围。
Phenotypic analysis of the medullary-type CD4+ CD8- [CD4 single-positive (SP)] thymocytes has revealed phenotypic heterogeneity within this cell population. The characteristic phenotype of mature peripheral T cells can be uniquely marked as Qa-2+ HSA- CD69-, whereas in the medullary-type CD4 SP thymocytes, the expression patterns of many markers were quite different. This suggests that there are many subgroups in the population, which reflects that medullary-type CD4 SP thymocytes may undergo phenotypic maturation. According to the results of two-colour flow cytometry, seven discrete phenotypes were identified by the expression capacity of Qa-2, HSA, CD69, 3G11 and 6C10 molecules. Consequently, the phenotypic precursor-progeny relationship can be envisaged as: 3G11- 6C10+ CD69+ HSAhi -->3G11+ 6C10+ CD69+ HSAhi --> 3G11+ 6C10- CD69+ HSAint -->3G11+ 6C10- CD69- HSAint Qa-2- -->3G11+ HSA-/lo Qa-2lo. At the stage of 3G11+ 6C10- CD69- HSAint Qa-2-, a branch pathway could be initiated, which gave rise to 3G11- HSAl degrees Qa-2- cells, which then, in turn, developed into 3G11- HSA-/loQa-2hi cells, a minor subgroup of the most mature CD4 SP cells. Consistent with this predicted pathway, experiments indicated that the first two subgroups were still cortisone sensitive, whereas the others were cortisone resistant. The cells in the last two Qa-2-positive subgroups are probably ready for emigration into the periphery.