Persistent conditioned place preference to aggression experience in adult male sexually-experienced CD-1 mice.
Persistent conditioned place preference to aggression experience in adult male sexually-experienced CD-1 mice.
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成年雄性有性经历的 CD-1 小鼠对攻击经历的持续条件性位置偏好。
DOI：
10.1111/gbb.12310
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发表时间：
2017-01
期刊：
影响因子：
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通讯作者：
Shaham Y
中科院分区：
文献类型：
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作者：
Golden SA;Aleyasin H;Heins R;Flanigan M;Heshmati M;Takahashi A;Russo SJ;Shaham Y
我们最近开发了一种条件性位置偏好 (CPP) 程序，通常用于研究奖励性药物效应，以证明具有性经验的优势 CD-1 雄性小鼠在先前与击败从属雄性 C57BL/6J 小鼠相关的环境中形成 CPP。在这里，我们进一步表征了 CD-1 小鼠的条件性和非条件性攻击行为。在Exp。在图 1 中，我们使用 CD-1 小鼠，这些小鼠对年轻的从属 C57BL/6J 入侵小鼠表现出多种无条件攻击行为。然后，我们在 CPP 程序中训练 CD-1 小鼠，其中一种环境是入侵者配对，而另一种环境则不是。然后我们在训练后 1 天测试攻击性 CPP。在Exp。如图2所示，我们在训练后1天和18天测试了CD-1小鼠的攻击性CPP。在Exp。如图 3-4 所示，我们训练 CD-1 小鼠通过杠杆按压来获取可口的食物，并测试它们对足部电击惩罚诱导的食物强化反应的抑制。在Exp。如图 5 所示，我们表征了杂种 CD-1xC57BL/6J D1-Cre 或 D2-Cre F1 代杂交中的无条件攻击。在 CD-1 小鼠中观察到持续攻击性 CPP，这些小鼠要么在所有筛选过程中立即攻击 C57BL/6J 小鼠，要么在筛选阶段逐渐出现攻击行为的小鼠。相比之下，在筛选过程中没有攻击 C57BL/6J 小鼠的 CD-1 小鼠不会在之前与 C57BL/6J 小鼠配对的环境中发展出 CPP。攻击性表型并不能预测对惩罚引起的食物强化反应抑制的抵抗力。 CD-1xD1-Cre 或 D2-Cre F1 转基因小鼠表现出强烈的无条件攻击性。我们的研究表明，攻击性经历会导致持续性 CPP，并引入转基因小鼠进行攻击性回路研究。
We recently developed a conditioned place preference (CPP) procedure, commonly used to study rewarding drug effects, to demonstrate that dominant sexually-experienced CD-1 male mice form CPP to contexts previously associated with defeating subordinate male C57BL/6J mice. Here we further characterized conditioned and unconditioned aggression behavior in CD-1 mice. In Exp. 1 we used CD-1 mice that displayed a variable spectrum of unconditioned aggressive behavior toward younger subordinate C57BL/6J intruder mice. We then trained the CD-1 mice in the CPP procedure where one context was intruder-paired, while a different context was not. We then tested for aggression CPP 1 day after training. In Exp. 2, we tested CD-1 mice for aggression CPP 1 day and 18 days after training. In Exp. 3–4, we trained the CD-1 mice to lever-press for palatable food and tested them for footshock punishment-induced suppression of food-reinforced responding. In Exp. 5, we characterized unconditioned aggression in hybrid CD-1xC57BL/6J D1-Cre or D2-Cre F1 generation crosses. Persistent aggression CPP was observed in CD-1 mice that either immediately attacked C57BL/6J mice during all screening sessions or mice that gradually developed aggressive behavior during the screening phase. In contrast, CD-1 mice that did not attack the C57BL/6J mice during screening didn’t develop CPP to contexts previously paired with C57BL/6J mice. The aggressive phenotype did not predict resistance to punishment-induced suppression of food-reinforced responding. CD-1xD1-Cre or D2-Cre F1 transgenic mice showed strong unconditioned aggression. Our study demonstrates that aggression experience causes persistent CPP and introduces transgenic mice for circuit studies of aggression.