THE EFFECT OF TUMOR NECROSIS FACTOR-ALPHA AND INTERFERON-GAMMA ON NEUTROPHIL FUNCTION
THE EFFECT OF TUMOR NECROSIS FACTOR-ALPHA AND INTERFERON-GAMMA ON NEUTROPHIL FUNCTION
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DOI：
10.1016/0022-4804(89)90195-9
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发表时间：
1989-04-01
影响因子：
2.2
通讯作者：
MALANGONI, MA
中科院分区：
文献类型：
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作者：
LIVINGSTON, DH;APPEL, SH;MALANGONI, MA
肿瘤坏死因子-α (TNF)和干扰素-γ (IFN-γ)已被证明可以调节细胞介导的免疫，并作为免疫功能的有效调节剂；然而，它们对中性粒细胞（PMN）功能的影响尚不清楚。本研究探讨了这些细胞因子对PMN吞噬、呼吸爆发和补体受体（C3b）表达的影响。人柠檬酸全血与磷酸盐缓冲盐水（对照）、20 μ大肠杆菌多糖（LPS）、TNF（1、10或100单位）或IFN-γ（1、10或100单位）孵育。还评估了TNF与IFN-γ之间的协同作用。用双醋酸二氯荧光素和标记金黄色葡萄球菌连续孵育血液，观察吞噬作用和呼吸爆发。采用标记抗cr3单克隆抗体检测C3b受体表达。测量结果表示为流式细胞术计数的2000个pmn的平均通道荧光。所有剂量的IFN-γ单独对测量的任何参数都没有影响。TNF - 1单位/ml吞噬能力（666±47比542±19）、呼吸爆发（326±33比258±17）、C3b（374±42比157±14）均高于对照组（p < 0.05）。TNF也表现出剂量依赖性的PMN激活。与单独使用任何一种药物相比，TNF + IFN-γ联合使用可增加呼吸爆发和C3b。这些数据表明TNF增强PMN功能，细胞因子相互作用可能在PMN激活中起重要作用。
Tumor necrosis factor-α (TNF) and interferon-γ (IFN-γ) have been shown to regulate cell-mediated immunity and act as effective modifiers of immune function; however, their influence on neutrophil (PMN) function is not well defined. This study investigated the effect of these cytokines on PMN phagocytosis, respiratory burst, and complement receptor (C3b) expression. Human citrated whole blood was incubated with either phosphate-buffered saline (control), 20 μgEscherichia colilipopolysaccharide (LPS), TNF (1, 10, or 100 units), or IFN-γ (1, 10, or 100 units). Synergy was also assessed between TNF and IFN-γ. Phagocytosis and respiratory burst were assayed by sequential incubation of blood with dichlorofluorescin diacetate followed by labeledStaphylococcus aureus. C3b receptor expression was assayed by labeled anti-CR3 monoclonal antibody. Measurements were expressed as the mean channel fluorescence of 2000 PMNs counted by flow cytometry. IFN-γ alone at all doses had no effect on any of the parameters measured. TNF 1 unit/ml increased phagocytosis (666 ± 47 vs 542 ± 19), respiratory burst (326 ± 33 vs 258 ± 17), and C3b (374 ± 42 vs 157 ± 14; allP< 0.05) over those of control. TNF also demonstrated dose-dependent PMN activation. The combination of TNF + IFN-γ increased both respiratory burst and C3b compared to either agent alone. These data indicate that TNF enhances PMN function and cytokine interaction may be important in PMN activation.