HOS15 and HDA9 negatively regulate immunity through histone deacetylation of intracellular immune receptor NLR genes in Arabidopsis.
HOS15 and HDA9 negatively regulate immunity through histone deacetylation of intracellular immune receptor NLR genes in Arabidopsis.
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DOI：
10.1111/nph.16380
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发表时间：
2019-12
期刊：
影响因子：
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通讯作者：
Leiyun Yang;Xiangsong Chen;Zhixue Wang;Qi Sun;Anna Hong;Aiqin Zhang;Xuehua Zhong;J. Hua
中科院分区：
文献类型：
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作者：
Leiyun Yang;Xiangsong Chen;Zhixue Wang;Qi Sun;Anna Hong;Aiqin Zhang;Xuehua Zhong;J. Hua
·植物免疫反应需要严格控制，以实现生长-防御平衡。这种严格控制背后的机制尚未完全了解。在这里，我们确定的表观遗传调控的核苷酸结合亮氨酸丰富的重复或Nod-Like受体（NLR）基因作为一个重要的免疫反应机制。通过致敏遗传筛选和分子研究，我们鉴定并表征了HOS 15及其相关蛋白HDA 9作为免疫和NLR基因表达的负调节因子。HOS 15或HDA 9的功能丧失赋予对病原体感染的增强的抗性，伴随着拟南芥中207个NLR基因中的三分之一的表达增加。HOS 15和HDA 9与这些NLR基因中的一些物理相关，并可能通过减少这些基因座处H3 K9的乙酰化来抑制它们的表达。此外，这些NLR基因在致病性和非致病性条件下均被HOS 15抑制，但仅在感染条件下被HDA 9抑制。·总之，这项研究揭示了植物免疫中以前未表征的组蛋白脱乙酰酶复合物，并强调了NLR基因的表观遗传调节在调节生长-防御平衡中的重要性。
•Plant immune responses need to be tightly controlled for growth-defense balance. The mechanism underlying this tight control is not fully understood. Here we identify epigenetic regulation of nucleotide-binding leucine rich repeat or Nod-Like Receptor (NLR) genes as an important mechanism for immune responses. •Through a sensitized genetic screen and molecular studies, we identified and characterized HOS15 and its associated protein HDA9 as negative regulators of immunity and NLR gene expression. •The loss-function of HOS15 or HDA9 confers enhanced resistance to pathogen infection accompanied with increased expression of one-third of the 207 NLR genes in Arabidopsis thaliana. HOS15 and HDA9 are physically associated with some of these NLR genes and repress their expression likely through reducing the acetylation of H3K9 at these loci. In addition, these NLR genes are repressed by HOS15 under both pathogenic and non-pathogenic conditions but by HDA9 only under infection condition. •Together, this study uncovers a previously uncharacterized histone deacetylase complex in plant immunity and highlights the importance of epigenetic regulation of NLR genes in modulating growth-defense balance.