A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement
A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement
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用于蛋白质弛豫增强测量的颓酸 Gd(III) 配位顺磁共溶剂
DOI：
10.1007/s10858-014-9817-3
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发表时间：
2014-03-01
影响因子：
2.7
通讯作者：
Tang, Chun
中科院分区：
文献类型：
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作者：
Gu, Xin-Hua;Gong, Zhou;Tang, Chun
溶剂顺磁弛豫增强（sPRE）产生于顺磁共溶剂和感兴趣的蛋白质之间的随机碰撞。sPRE可以容易地测量，提供蛋白质结构信息。然而，缺乏惰性共溶剂探针可能产生与蛋白质结构不一致的sPRE值。在这里，我们合成了一种新的sPRE探针，三亚乙基四胺六乙酸三甲基酰胺钆，或Gd（III）-TTHA-TMA。由于共有10个配位位点，这种顺磁性共溶剂消除了一个内球水分子，否则该水分子可以通过交换将弛豫转移到蛋白质。在金属离子居中的情况下，新探针主要是半径为4.0 μ m的球形，允许精确反算sPRE。Gd（III）-TTHA-TMA作为sPRE探针的有效性在三种充分研究的蛋白质系统上得到证明。
Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)-TTHA-TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 Å, permitting accurate back calculation of sPRE. The effectiveness Gd(III)-TTHA-TMA as a sPRE probe was demonstrated on three well-studied protein systems.