Biosynthesis of Xyloglucan in Suspension-Cultured Soybean Cells. Synthesis of Xyloglucan from UDP-Glucose and UDP-Xylose in the Cell-Free System :
Biosynthesis of Xyloglucan in Suspension-Cultured Soybean Cells. Synthesis of Xyloglucan from UDP-Glucose and UDP-Xylose in the Cell-Free System :
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悬浮培养的大豆细胞中木葡聚糖的生物合成。
DOI：
10.1093/oxfordjournals.pcp.a076194
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发表时间：
1981
影响因子：
4.9
通讯作者：
K. Matsuda
中科院分区：
文献类型：
--
作者：
Takahisa Hayashi;K. Matsuda
当UDP-[14 C]葡萄糖或UDP-[14 C]木糖与来自大豆细胞的颗粒部分一起孵育时，合成了放射性聚合物。在用米曲霉酶消化时，这些聚合物得到14 C-单糖和14 C-二糖，其色谱和电泳迁移率与真正的异苦木糖（6-O-α-D-吡喃木糖基-D-吡喃葡萄糖）的迁移率没有区别。二糖由木糖和葡萄糖组成，葡萄糖位于还原端。通过甲基化分析提供了二糖是异苦木二糖的证据。水解甲基化二糖产生2，3，4-三-O-甲基-D-木糖和2，3，4-三-O-甲基-D-葡萄糖。因此，将放射性掺入到木葡聚糖的最小结构单元异苦木糖中，表明木葡聚糖在体外由UDP-葡萄糖和UDP-木糖合成。
When UDP-[14C]glucose or UDP-[14C]xylose was incubated with a particulate fraction from soybean cells, radioactive polymers were synthesized. On digestion withAspergillus oryzaeenzymes, these polymers gave14C-monosaccharides and a14C-disaccharide with chromatographic and electrophoretic mobilities indistinguishable from those of authentic isoprimeverose (6-O-α-D-xylopyranosyl-D-glucopyranose). The disaccharide consisted of xylose and glucose, and the latter was located at the reducing end. Evidence that the disaccharide is isoprimeverose was provided by methylation analysis. Hydrolysis of the methylated disaccharide yielded 2,3,4-tri-O-methyl-D-xylose and 2,3,4-tri-O-methyl-D-glucose. Thus, incorporation of radioactivity into isoprimeverose, the smallest structural unit of xyloglucan, suggests that xyloglucan is synthesized in vitro from UDP-glucose and UDP-xylose.