Tumorigenicity, immunogenicity, and virus production in mouse melanoma cells treated with 5-bromodeoxyuridine.
Tumorigenicity, immunogenicity, and virus production in mouse melanoma cells treated with 5-bromodeoxyuridine.
复制标题
用 5-溴脱氧尿苷处理的小鼠黑色素瘤细胞的致瘤性、免疫原性和病毒产生。
DOI：
10.1073/pnas.69.11.3443
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发表时间：
1972
影响因子：
11.1
通讯作者：
E. Deharven
中科院分区：
文献类型：
--
作者：
S. Silagi;D. Beju;J. Wrathall;E. Deharven
溴脱氧尿苷（BrdU），无论是以30 μ g/ml的30小时脉冲给药还是以低浓度（1-3 μ g/ml）连续给药，都显著增加了小鼠黑素瘤（B16）细胞系中具有鼠白血病病毒形态的颗粒的产生。颗粒在对照细胞中非常罕见，只能通过电子显微镜检测。相比之下，在用BrdU处理的细胞进行的许多实验中，通过电子显微镜计数的病毒颗粒的数量增加了100倍以上，并且小鼠白血病病毒的其他测试（空斑试验和组特异性抗原1和3以及Gross细胞表面抗原的测试）变得阳性。所有BrdU处理的细胞，无论药物浓度或治疗时间长短，除了显示色素和堆积形态的损失，与对照细胞相比，在致瘤性方面受到抑制。这些影响都是可逆的。当用恶性对照细胞攻击时，注射BrdU处理的细胞的显著百分比的小鼠被保护免于随后的肿瘤形成。给予小鼠的保护程度与注射细胞中计数的病毒颗粒数量密切相关。对于每毫升1微克BrdU连续处理的细胞，细胞相关病毒的数量和恶性肿瘤的抑制程度之间也有很好的相关性，但对于高浓度BrdU短期处理的细胞，相关性就不那么好了。
Bromodeoxyuridine (BrdU), whether administered in a 30-hr pulse of 30 mug/ml or continuously in low concentrations (1-3 mug/ml), significantly increased production of particles with the morphology of murine leukemia virus in a mouse melanoma (B16) cell line. Particles were very rare in control cells, detectable only by electron microscopy. By contrast, in many experiments with BrdU-treated cells the numbers of virus particles counted by electron microscopy increased over 100-fold, and other tests for murine leukemia virus (plaque assay and tests for group-specific antigens 1 and 3 and for Gross cell-surface antigens) became positive. All BrdU-treated cells, regardless of drug concentration or length of treatment, in addition to showing loss of both pigment and of piled-up morphology, were suppressed in tumorigenicity compared with the control cells. These effects were all reversible. A significant percentage of mice injected with BrdU-treated cells were protected against subsequent tumor formation when challenged with malignant control cells. The degree of protection conferred on the mice correlated well with the number of virus particles counted in the injected cells. There was also good correlation between the amount of cell-associated virus and the degree of suppression of malignancy for cells treated continuously with 1 mug of BrdU per ml, but not as good for cells treated for short periods with higher concentrations of BrdU.