Population genetic analysis of gray’s pipefish, Halicampus grayi in the South China Sea
Population genetic analysis of gray’s pipefish, Halicampus grayi in the South China Sea
复制标题
南海灰龙鱼种群遗传分析
DOI：
10.1007/s13258-019-00893-9
复制
发表时间：
2019
影响因子：
2.1
通讯作者：
Lin Qiang
中科院分区：
文献类型：
--
作者：
Wu Yingying;Wang Xin;Liu Shuaishuai;Luo Hao;Lin Qiang
BackgroundThe evolution of male pregnancy is the most distinctive characteristic of syngnathids, and they were recognized as flagship species for marine conservation. GenusHalicampusis an important branch of syngnathid fishes that has not received the attention it deserves.ObjectiveTo sequence the mitochondrial genome ofHalicampus grayi, and investage the genetic structure of its populations.MethodsDegenerate primers were designed to amplify the entire mitochondrial genome ofH. grayi. The phylogenetic relationship betweenH. grayiand other syngnathids were conducted using maximum-likelihood method. Population genetic structure of three geographic population ofH. grayiwere determined using median-joining haplotype network based on COI and Cytb sequences.ResultsThe complete mitochondrial genome ofHalicampus grayiwas assembled into a 17,059 bp circular sequence, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 D-loop region. The overall base composition ofH. grayiis 29.93% A, 29.31% T, 16.23% G and 24.54% C, with a slight A + T rich feature (59.24%). Phylogenetic analysis indicated thatH. grayihas a close relationship withTrachyrhamphus serratus. Population genetic analysis revealed a relatively high genetic diversity across different geographic populations ofH. grayi, and the results of median-joining haplotype network indicated a lack of structure in populations ofH. grayi.ConclusionThe mitogenome ofH. grayiwill provided important information about the origin and evolution issues of syngnathid fishes, and the high-level genetic diversity detected in their populations will provide insight into the gene flow pattern of marine fishes.
