Improved method for quantitative determination in serum of alkaline phosphatase of skeletal origin.
Improved method for quantitative determination in serum of alkaline phosphatase of skeletal origin.
复制标题
血清中骨骼来源碱性磷酸酶定量测定的改进方法。
DOI：
10.1093/clinchem/27.12.2002
复制
发表时间：
1981
影响因子：
9.3
通讯作者：
D. Baylink
中科院分区：
文献类型：
--
作者：
J. Farley;C. Chesnut;D. Baylink
在检测人血清中骨骼碱性磷酸酶（EC 3.1.3.1）活性的定量方法中，通过对L-苯丙氨酸抑制的敏感性识别肠和胎盘碱性磷酸酶活性，通过对热灭活的不同敏感性区分骨骼和肝碱性磷酸酶。从器官来源制备的碱性磷酸酶同工酶可能与血清中相应的同工酶表现不同。我们的程序允许我们包括骨骼，肠道和胆汁碱性磷酸酶的器官来源的内部标准，以尽量减少测定之间的变化。在初步应用中，我们发现（a）总血清碱性磷酸酶活性在绝经后骨质疏松受试者中变化极大，并不是骨骼碱性磷酸酶活性的可靠指标;（B）与年龄匹配的对照组相比，7名对氟化钠治疗有反应的骨质疏松患者的骨形成增加，其血清中的骨骼碱性磷酸酶活性增加（p小于0.005）;和（c）10名对司坦唑醇治疗有反应且全身钙增加的绝经后骨质疏松患者显示循环骨骼碱性磷酸酶活性增加（p小于0.001）。
In this quantitative method for detection of skeletal alkaline phosphatase (EC 3.1.3.1) activity in human serum, intestinal and placental alkaline phosphatase activities are recognized by their susceptibility to inhibition by L-phenylalanine, and skeletal and hepatic alkaline phosphatases are distinguished by their different sensitivities to inactivation by heat. Alkaline phosphatase isoenzymes prepared from organ sources may behave differently from the corresponding isoenzymes in serum. Our procedure allows us to include organ-derived internal standards of skeletal, intestinal, and biliary alkaline phosphatase to minimize between-assay variation. In preliminary applications, we have found that (a) total serum alkaline phosphatase activity is extremely variable in post-menopausal osteoporotic subjects and is not a reliable index of skeletal alkaline phosphatase activity; (b) seven osteoporotic patients responding to therapy with sodium fluoride with increased bone formation showed increased skeletal alkaline phosphatase activity in their serum as compared with age-matched controls (p less than 0.005); and (c) 10 post-menopausal osteoporotic patients responding to therapy with stanozolol with increased total body calcium showed an increase in circulating skeletal alkaline phosphatase activity (p less than 0.001).