An ultra-sensitive and specific nanoplasmonic-enhanced isothermal amplification platform for the ultrafast point-of-care testing of SARS-CoV-2.
An ultra-sensitive and specific nanoplasmonic-enhanced isothermal amplification platform for the ultrafast point-of-care testing of SARS-CoV-2.
复制标题
超灵敏、特异的纳米等离子增强等温扩增平台，用于 SARS-CoV-2 的超快现场护理测试。
DOI：
10.1016/j.cej.2022.138822
复制
发表时间：
2023-01-01
影响因子：
15.1
通讯作者：
Liu, Gang L.
中科院分区：
文献类型：
--
作者：
Liu, Juxiang;Chen, Ping;Hu, Xulong;Huang, Liping;Geng, Zhi;Xu, Hao;Hu, Wenjun;Wang, Lin;Wu, Ping;Liu, Gang L.
关键词：
SARS-COV-2的高诱变使其预防和治疗的挑战归因于超快速的挑战。新型策略提供了一个易于操作的检测平台，可用于在CT值<25的临床样本中获得超快速和高通量的数据（96个样本）使用来自21例SARS-COV-2感染患者的临床样本和31个健康的患者进行了验证。 n基因和ORF1AB基因的92.3％和91.7％分别为28.3副本/mL和23.3份和23.3副本/ml，用于N Gene和ORF1AB的范围，并备有有效的Nananopeia检测策略范围。开关的地区。
The novel mutations attributed by the high mutagenicity of the SARS-CoV-2 makes its prevention and treatment challenging. Developing an ultra-fast, point-of-care-test (POCT) protocol is critical for responding to large-scale spread of SARS-CoV-2 in public places and in resource-poor remote areas. Here, we developed a nanoplasmonic enhanced isothermal amplification (NanoPEIA) strategy that combines a nanoplasmonic sensor with isothermal amplification. The novel strategy provides an ideal easy-to operate detection platform for obtaining accurate, ultra-fast and high-throughput (96 samples can be tested together) data. For clinical samples with viral detection at Ct value <25, the entire process (including sample preparation, virus lysis, detection, and data analysis) can be completed within six minutes. The method is also appropriate for detection of SARS-CoV-2 γ-coronavirus mutants. The NanoPEIA method was validated using clinical samples from 21 patients with SARS-CoV-2 infection and 31 healthy individuals. The detection result on the 52 clinical samples for SARS-CoV-2 showed that the NanoPEIA platform had a 100% sensitivity for N and orf1ab genes, which was higher than those obtained using RT-qPCR (88.9% and 90.0%, respectively). The specificities of 31 clinical negative samples were 92.3% and 91.7% for the N gene and the orf1ab gene, respectively. The limits of detection (LoD) of the clinical samples were 28.3 copies/mL and 23.3 copies/mL for the N gene and the orf1ab gene, respectively. The efficient NanoPEIA detection strategy facilitates real-time detection and visualization within ultrashort durations and can be applied for POCT diagnosis in resource-poor and highly populated areas.
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