Grapevine immune signaling network in response to drought stress as revealed by tray scriptomic analysis
Grapevine immune signaling network in response to drought stress as revealed by tray scriptomic analysis
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托盘脚本组分析揭示了葡萄树响应干旱胁迫的免疫信号网络
DOI：
10.1016/j.plaphy.2017.10.026
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发表时间：
2017-12-01
影响因子：
6.5
通讯作者：
Fang, Jinggui
中科院分区：
文献类型：
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作者：
Haider, Muhammad S.;Kurjogi, Mahantesh M.;Fang, Jinggui
干旱是一种普遍存在的非生物因素，严重阻碍了园艺作物的生长发育。全球气候变化带来的挑战是能够应对同时逆境的耐旱品种的进化。因此，本研究对干旱处理的葡萄叶片进行了生化、生理和转录组分析。结果表明，干旱胁迫下叶片的光合作用活性和还原糖含量显着降低，与气孔导度和CO2交换能力降低呈正相关。此外，在干旱响应的葡萄叶片中，超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性被显著激活。同样，在干旱胁迫下，叶片中脱落酸和茉莉酸的含量也显著增加。在转录组分析中，共注释了12,451个差异表达基因，其中8021个差异表达基因表达上调，4430个差异表达基因表达下调。此外，编码病原体相关分子模式(PAMP)的基因触发免疫(PTI)，包括钙信号、蛋白磷酸酶2C、类钙调神经磷酸酶B蛋白、MAPKs和磷酸化(Fls2和MEKK1)级联基因在干旱胁迫下表达上调。与植物-病原菌相互作用途径相关的几个基因(RPM1、PBS1、RPS5、RIN4、MIN7、PR1和WRKYs)也在干旱胁迫下表达上调。综上所述，本研究结果显示了DEG在葡萄生理上的动态互作，为后续葡萄育种提供了筛选抗旱相关基因的前提。
Drought is a ubiquitous abiotic factor that severely impedes growth and development of horticulture crops. The challenge postured by global climate change is the evolution of drought-tolerant cultivars that could cope with concurrent stress. Hence, in this study, biochemical, physiological and transcriptome analysis were investigated in drought-treated grapevine leaves. The results revealed that photosynthetic activity and reducing sugars were significantly diminished which were positively correlated with low stomatal conductance and CO2 exchange in drought-stressed leaves. Further, the activities of superoxide dismutase, peroxidase, and catalase were significantly actuated in the drought-responsive grapevine leaves. Similarly, the levels of abscisic acid and jasmonic acid were also significantly increased in the drought-stressed leaves. In transcriptome analysis, 12,451 differentially-expressed genes (DEGs) were annotated, out of which 8021 DEGs were up-regulated and 4430 DEGs were down-regulated in response to drought stress. In addition, the genes encoding pathogen-associated molecular pattern (PAMP) triggered immunity (PTI), including calcium signals, protein phosphatase 2C, calcineurin B-like proteins, MAPKs, and phosphorylation (FLS2 and MEKK1) cascades were up-regulated in response to drought stress. Several genes related to plant-pathogen interaction pathway (RPM1, PBS1, RPS5, RIN4, MIN7, PR1, and WRKYs) were also found up-regulated in response to drought stress. Overall the results of present study showed the dynamic interaction of DEG in grapevine physiology which provides the premise for selection of defense-related genes against drought stress for subsequent grapevine breeding programs.