Inheritance and interactions of incompatibility alleles in the tetraploid sour cherry
Inheritance and interactions of incompatibility alleles in the tetraploid sour cherry
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DOI：
10.1007/s00122-005-0130-0
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发表时间：
2006-01-01
影响因子：
5.4
通讯作者：
Sonneveld, T
中科院分区：
文献类型：
--
作者：
Boskovic, RI;Wolfram, B;Sonneveld, T
分析了甜樱桃与甜樱桃异源四倍体杂种的3个酸樱桃后代的自交亲和性与S核糖核酸酶表型的相关性。自交后在田间和通过监测花粉管生长来鉴定自交(自交)亲和性。花柱蛋白等电聚焦和核糖核酸酶活性染色确定S核糖核酸酶表型，亲本用聚合酶链式反应确认表型。苗期表型基本符合S核糖核酸酶等位基因的二体分离。亲本的遗传安排为：‘Koroser’(自交不亲和)S-1 S-4.SBSD，‘Schtenmorelle’(自交不亲和)S6S13.SBSB，43.87(自交不亲和)S4S13.SBSB，其中“。分离出两个同源的基因组。S-4和S-6等位基因位于同一座位导致自交不亲和，而S-13和S-B位于同源座位则导致自交不亲和。在三个杂交组合或自交不亲和苗木中，某些异等位基因均失败，表明S-4和S-6对S-B是显性的。然而，S-13 S-B花粉在花柱上成功地表达了相应的S-核糖核酸酶，这表明竞争互作或缺乏花粉-S成分。总体而言，S-13和S-B花粉的普遍亲和性可能是S-13和S-B一起出现在酸樱桃品种中的原因。对可能来自磨碎樱桃的等位基因S-B和S-D以及可能来自甜樱桃的S-13进行了测序。我们的发现有助于理解自交(内)亲和性的遗传，便于自交亲和性后代的筛选，并为研究异等位花粉的分子互作提供了基础。
Three progenies of sour cherry (Prunus cerasus) were analysed to correlate self-(in)compatibility status with S-RNase phenotype in this allotetraploid hybrid of sweet and ground cherry. Self-(in)compatibility was assessed in the field and by monitoring pollen tube growth after selfing. The S-RNase phenotypes were determined by isoelectric focusing of stylar proteins and staining for RNase activity and, for the parents, confirmed by PCR. Seedling phenotypes were generally consistent with disomic segregation of S-RNase alleles. The genetic arrangements of the parents were deduced to be 'Koroser' (self-incompatible) S-1 S-4 .SBSD , 'Schattenmorelle' (self-compatible) S6S13.SBSB, and clone 43.87 (self-compatible) S4S13.SBSB , where "." separates the two homoeologous genomes. The presence of S-4 and S-6 alleles at the same locus led to self-incompatibility, whereas S-13 and S-B at homoeologous loci led to self-compatibility. The failure of certain heteroallelic genotypes in the three crosses or in the self-incompatible seedlings indicates that S-4 and S-6 are dominant to S-B . However, the success of S-13 S-B pollen on styles expressing corresponding S-RNases indicates competitive interaction or lack of pollen-S components. In general, the universal compatibility of S-13 S-B pollen may explain the frequent occurrence of S-13 and S-B together in sour cherry cultivars. Alleles S-B and S-D , that are presumed to derive from ground cherry, and S-13 , presumably from sweet cherry, were sequenced. Our findings contribute to an understanding of inheritance of self-(in)compatibility, facilitate screening of progenies for self-compatibility and provide a basis for studying molecular interactions in heteroallelic pollen.