Luteolin Showed a Resistance Elimination Effect on Gentamicin by Decreasing MATE mRNA Expression in Trueperella pyogenes
Luteolin Showed a Resistance Elimination Effect on Gentamicin by Decreasing MATE mRNA Expression in Trueperella pyogenes
复制标题
木犀草素通过降低化脓性 Trueperella 中 MATE mRNA 的表达，显示出对庆大霉素耐药性的消除作用
DOI：
10.1089/mdr.2018.0097
复制
发表时间：
2018-11-15
影响因子：
2.6
通讯作者：
Liu, Mingchun
中科院分区：
文献类型：
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作者：
Zhang, Dexian;Gao, Xiang;Liu, Mingchun
化脓性真杆菌是动物粘膜表面的常见寄生菌，并在奶牛中引起多种感染，包括乳房炎、乳腺炎和肝衰竭。许多抗微生物剂用于治疗由T.化脓性链球菌;然而，抗生素耐药性最近已成为严重的问题。本研究的目的是表征外排泵编码的多药和毒性化合物外排（MATE）基因对T。化脓性链球菌分离自具有乳房炎体征的奶牛。木犀草素作为一种植物源化合物，对大肠杆菌和金黄色葡萄球菌具有抗菌活性，因此，我们也研究了木犀草素是否可以消除抗生素耐药性。我们在BM-H 06 -3中构建了MATE缺失突变体，以鉴定MATE在抗生素抗性中的功能。通过检测MATE mRNA的表达，探讨木犀草素消除T.化脓霸王MATE缺失后，化脓性链球菌分离株BM-H 06 -3变得对庆大霉素、阿米卡星、链霉素、红霉素和罗红霉素敏感。从34株T.结果表明，毛地黄黄酮对庆大霉素敏感，但在1/4最低抑菌浓度（MIC）下作用36小时后，对庆大霉素敏感。此外，毛地黄黄酮可以降低MATE mRNA的表达后，毛地黄黄酮处理在亚抑制浓度（1/4 MIC）。我们发现MATE基因与抗生素耐药性有关，木犀草素可诱导T.化脓因此，毛地黄黄酮可能是一种潜在的抑制多药耐药T细胞外排泵的药物。化脓
Trueperella pyogenes is a common inhabitant of mucosal surfaces in animals and causes a variety of infections, including endometritis, mastitis, and liver abscessation, in dairy cows. Many antimicrobial agents are used for treatment of infections caused by T. pyogenes; however, antibiotic resistance has recently become a serious problem. The objective of this study was to characterize the effect of the efflux pump-encoding multidrug and toxic compound extrusion (MATE) gene on antibiotic resistance in T. pyogenes isolates from cows with signs of endometritis. As a compound from plants, luteolin showed antimicrobial activities in Escherichia coli and Staphylococcus aureus; therefore, we also investigated whether luteolin can eliminate antibiotic resistance. We constructed a MATE deletion mutant in BM-H06-3 to identify the function of MATE in antibiotic resistance. MATE mRNA expression was measured to identify the mechanism of luteolin in gentamicin resistance elimination effect in T. pyogenes. The T. pyogenes isolate BM-H06-3 became susceptible to gentamicin, amikacin, streptomycin, erythromycin, and roxithromycin after MATE deletion. No synergistic effect between luteolin and gentamicin was observed in eight isolates, which were randomly selected from 34 T. pyogenes isolates, but the isolates became susceptible to gentamicin after luteolin treatment at a subinhibitory concentration (1/4 minimum inhibitory concentration [MIC]) for 36hr. Furthermore, luteolin can decrease MATE mRNA expression after luteolin treatment at a subinhibitory concentration (1/4 MIC). We found that the MATE gene was involved in antibiotic resistance and that luteolin induces a resistance elimination effect in T. pyogenes. Therefore, luteolin may be a potential agent to inhibit efflux pumps in multidrug-resistant T. pyogenes.