Transcriptome-wide survey and expression analysis of stress-responsive NAC genes in Chrysanthemum lavandulifolium
Transcriptome-wide survey and expression analysis of stress-responsive NAC genes in Chrysanthemum lavandulifolium
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薰衣草胁迫响应 NAC 基因的全转录组调查和表达分析
DOI：
10.1016/j.plantsci.2012.05.004
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发表时间：
2012-09-01
期刊：
影响因子：
5.2
通讯作者：
Dai, Silan
中科院分区：
文献类型：
--
作者：
Huang, He;Wang, Yi;Dai, Silan
植物特异性NAC（NAM、ATAF和CUC）转录因子家族在多种植物生长发育过程以及逆境抗性中起着重要作用。利用RNA测序技术，我们发现CINAC基因（CINAC 1 -44）是盐胁迫下菊花叶片中表达上调最强的转录因子家族。我们进行了逆转录聚合酶链反应，以监测CINAC基因对多种胁迫和激素处理，包括盐，干旱，寒冷，炎热，脱落酸和水杨酸处理的反应。结果表明，有35个CINAC基因在不同器官中存在差异表达，其中32个CINAC基因至少对2种处理有反应。真实的实时定量聚合酶链反应结果表明，7个不同亚家族的10个CINAC基因至少对5种处理有反应。在6种不同类型的处理下，观察到CINAC 17和CINAC 21基因的转录水平的超过50倍的变化。在本研究中，CINAC基因在非生物胁迫和激素处理下的高水平表达表明NAC转录因子在非生物胁迫耐受和适应中起重要作用。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
The plant-specific NAC (NAM, ATAF, and CUC) transcription factor family plays a vital role in various plant growth and developmental processes as well as in stress resistance. Using RNA sequencing, we found that the CINAC genes (CINAC1-44) were the most strongly up-regulated transcription factor family in Chrysanthemum layandulifolium leaves under salt treatment. We carried out reverse transcriptase polymerase chain reaction to monitor CINAC genes response against multiple stresses and hormonal treatments including salt, drought, cold, heat, abscisic acid and salicylic acid treatments. The results showed that 35 CINAC genes were differentially expressed in different organ, and 32 CINAC genes could respond to at least 2 kinds of treatments. Quantitative real time polymerase chain reaction showed that 10 CINAC genes belonging to 7 different subfamilies could respond to at least 5 kinds of treatments. Over 50-fold variation in transcriptional levels of CINAC17 and CINAC21 genes was observed under 6 different types of treatments. In the present study, high-level expression of CINAC genes under abiotic stresses and hormonal treatments suggests that the NAC transcription factors play important roles in abiotic stress tolerance and adaptation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.