A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis
A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis
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Z染色体拓扑结构的重排影响欧洲玉米螟Ostrinia nubilalis的性连锁基因展示
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发表时间：
2011
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通讯作者：
R. Hellmich
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作者：
Jeremy A. Kroemer;Brad S. Coates;T. Nusawardani;S. Dean Rider;Lisa M. Fraser;R. Hellmich
就许多蝴蝶和飞蛾物种（鳞翅目昆虫）的性染色体而言，雄性是同配的（ZZ），雌性是异配的（WZ）。 Z 染色体上的基因影响幼虫发育、环境适应和生殖隔离等性状。为了促进对鳞翅目这些性状的研究，我们开发了 43 个简并引物对来 PCR 扩增 43 个家蚕 Z 染色体连锁基因的直向同源物。在玉米螟中通过 PCR 扩增的 34 个直向同源物中，有 6 个与 Z 染色体锚定标记 kettin (ket) 和乳酸脱氢酶 (ldh) 共分离，并与 5 个 AFLP 标记组合产生约 89 cM 的共有遗传连锁图。 O. nubilalis 和 B. mori Z 染色体相对共线，尽管潜在的基因倒位改变了末端基因顺序，并且易位事件破坏了一条染色体末端的同线性。与家蚕直系同源物相比，O. nubilalis Z染色体连锁基因显示出组织特异性和生长阶段特异性表达的保守性，尽管一些基因在整个发育阶段或组织中表现出物种特异性表达。 O. nubilalis Z染色体连锁图为分离参与驱动物种形成的性连锁性状的数量性状位点（QTL）提供了新工具，并揭示了基因组重排作为鳞翅目差异基因调控的可能机制。
Males are homogametic (ZZ) and females are heterogametic (WZ) with respect to the sex chromosomes in many species of butterflies and moths (insect order Lepidoptera). Genes on the Z chromosome influence traits involved in larval development, environmental adaptation, and reproductive isolation. To facilitate the investigation of these traits across Lepidoptera, we developed 43 degenerate primer pairs to PCR amplify orthologs of 43 Bombyx mori Z chromosome-linked genes. Of the 34 orthologs that amplified by PCR in Ostrinia nubilalis, 6 co-segregated with the Z chromosome anchor markers kettin (ket) and lactate dehydrogenase (ldh), and produced a consensus genetic linkage map of ~89 cM in combination with 5 AFLP markers. The O. nubilalis and B. mori Z chromosomes are comparatively co-linear, although potential gene inversions alter terminal gene orders and a translocation event disrupted synteny at one chromosome end. Compared to B. mori orthologs, O. nubilalis Z chromosome-linked genes showed conservation of tissue-specific and growth-stage-specific expression, although some genes exhibited species-specific expression across developmental stages or tissues. The O. nubilalis Z chromosome linkage map provides new tools for isolating quantitative trait loci (QTL) involved in sex-linked traits that drive speciation and it exposes genome rearrangements as a possible mechanism for differential gene regulation in Lepidoptera.