Food vacuole proteome of the malarial parasite Plasmodium falciparum
Food vacuole proteome of the malarial parasite Plasmodium falciparum
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DOI：
10.1002/prca.200700112
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发表时间：
2008-09-01
影响因子：
2
通讯作者：
Dubremetz, Jean-Francois
中科院分区：
文献类型：
--
作者：
Lamarque, Mauld;Tastet, Christophe;Dubremetz, Jean-Francois
恶性疟原虫食物液泡（FV）是一种溶酶体样细胞器，红细胞血红蛋白在其中消化。它是抗疟药物开发中最受欢迎的靶点。我们使用串联质谱法研究了fv富集部分的蛋白质组，并鉴定了116种蛋白质。电镜分析和Western blot数据显示，该部分的主要成分是FV，并且正如预期的那样，大多数先前已知的FV标记被恢复。特别有趣的是，研究人员发现了几种参与囊泡介导转运的蛋白质，这些蛋白质可能在FV生物发生和/或FV蛋白转运中发挥关键作用。寄生虫表面蛋白的恢复支持血红蛋白摄取的细胞造口途径作为FV运输途径。我们已经在数据库中确定了32种被描述为假设的蛋白质。这种对FV蛋白含量的了解为理解该细胞器在恶性疟原虫中的生物学功能提供了新的线索。
The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P.falciparum.