Analysis of the effects of mepiquat chloride priming on the seedling growth-promoting in cotton under salt stress by multi-omics
Analysis of the effects of mepiquat chloride priming on the seedling growth-promoting in cotton under salt stress by multi-omics
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DOI：
10.1016/j.indcrop.2022.115296
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发表时间：
2022-10
影响因子：
5.9
通讯作者：
Ning Wang;Xiangru Wang;Qian Qi;A. Iqbal;Hengheng Zhang;Jianbin Shi;Qiang Dong;Qinghuan Xu
中科院分区：
文献类型：
--
作者：
Ning Wang;Xiangru Wang;Qian Qi;A. Iqbal;Hengheng Zhang;Jianbin Shi;Qiang Dong;Qinghuan Xu
对棉苗进行了多组学研究，以确定助壮素 (MC) 启动在盐胁迫下对棉花幼苗的促进作用。 MC-priming markedly alleviated salt stress inhibition in root anatomical structures, increasing seedling establishment.比较多组学分析揭示了多种能量代谢途径是 MC 启动的关键影响因素，解释了盐胁迫的缓解。 MC 启动上调盐胁迫下三酰甘油 (TAG) 动员、γ-氨基丁酸 (GABA) 分流和三磷酸腺苷 (ATP) 合成的关键蛋白和磷酸化。共表达网络分析还表明，MC 启动以涉及耐盐反应的相互作用的方式调节这些代谢途径。重要的是，β-氧化和 GABA 合成的抑制剂破坏了盐胁迫下 MC 启动诱导的生长，证实了这些途径在形成 MC 启动反应中的重要性。 MC-priming significantly blocked the salt-triggered TAG accumulation and early enhanced GABA shunt activities under salt stress.同时，MC启动通过上调ATP合酶和下调细胞色素b5样还原酶来提高能量效率，从而增强ATP形成并减少活性氧的产生。 In addition, MC-priming enhanced antioxidant enzymatic activities under salt stress. These findings provide insights into the energy regulatory pathway of MC-priming that mediates growth-promotion under salt stress. Thus, this study reinforces the potential of MC-priming to improve cotton seed germination under salt stress.
A multi-omics study was conducted on cotton seedlings to determine the growth promotion caused by mepiquat chloride (MC)-priming under salt stress. MC-priming markedly alleviated salt stress inhibition in root anatomical structures, increasing seedling establishment. Comparative multi-omics analyses revealed various energy metabolic pathways as key influencers of MC-priming, explaining the alleviation of salt stress. MC-priming up-regulated key proteins and phosphorylation for triacylglycerol (TAG) mobilization, γ-aminobutyric acid (GABA) shunt, and adenosine triphosphate (ATP) synthesis under salt stress. The coexpression network analyses also suggested that MC-priming regulates these metabolic pathways in a mutual-functioning fashion involving salt tolerance responses. Importantly, the inhibitor of β-oxidation and GABA-synthesis disrupted the MC-priming-induced growth under salt stress, confirming the importance of these pathways in forming the MC-priming response. MC-priming significantly blocked the salt-triggered TAG accumulation and early enhanced GABA shunt activities under salt stress. Meanwhile, MC-priming improved energy efficiency by up-regulating ATP synthase and down-regulating cytochrome b5-like reductase, thereby enhancing ATP formation and reducing reactive oxygen species production. In addition, MC-priming enhanced antioxidant enzymatic activities under salt stress. These findings provide insights into the energy regulatory pathway of MC-priming that mediates growth-promotion under salt stress. Thus, this study reinforces the potential of MC-priming to improve cotton seed germination under salt stress.