Strongyloides ratti: thermokinetic behavior of third-stage larvae on a temperature gradient.
Strongyloides ratti: thermokinetic behavior of third-stage larvae on a temperature gradient.
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鼠类圆线虫：第三阶段幼虫在温度梯度上的热动力学行为。
DOI：
10.1006/expr.2000.4526
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发表时间：
2000
影响因子：
2.1
通讯作者：
Isao Tada
中科院分区：
文献类型：
--
作者：
H. Tobata;Masaaki Shimada;Masataka Koga;Isao Tada
采用琼脂糖跟踪法研究了啮齿动物寄生线虫侵染性第三期幼虫（L3）在温度梯度上的热动力学行为。观察到的行为包括消极和积极的热运动，运动方向取决于幼虫最初放置的梯度温度和先前的培养温度经验。从25摄氏度培养的大鼠粪便中分离出来的幼虫，在22摄氏度到29摄氏度之间的温度梯度上，倾向于向更高的温度移动。在较高的放置温度下，大多数幼虫移动很少，没有方向性响应，而在较低的放置温度下，许多幼虫向较低的温度迁移。在20摄氏度或以下的放置温度下，很少或没有幼虫向温度较高的区域移动。从20℃培养的大鼠粪便中分离出的幼虫，无论放置温度如何，都倾向于向高温区域迁移。在30℃下培养的细胞对温度梯度没有反应。在30℃条件下培养的L3对大鼠的感染性明显低于在25℃和20℃条件下培养的L3，另外设计了实验来证明L3期后的热动力学行为。在37℃双蒸馏水（DDW）中孵育24 h的幼虫对较低温度的反应丧失，而在15℃和25℃双蒸馏水中孵育的幼虫对较低温度的反应仍然明显。S. ratti L3的热动力学行为受周围环境温度的影响，这可能在寻找寄主中起重要作用。
We examined the thermokinetic behaviors of infective third-stage larvae (L3) of the rodent parasitic nematode Strongyloides ratti on temperature gradients using an in vitro agarose tracking assay method. Observed behaviors included both negative and positive thermokineses, the direction of movement depending both on the gradient temperature at which larvae were initially placed and on prior experience of culture temperature. Larvae isolated from rat feces cultured at 25 degrees C and placed on a gradient at temperatures between 22 degrees and 29 degrees C tended to move toward higher temperatures. At higher placement temperatures, most larvae moved little and showed no directional response, whereas at lower placement temperatures, many migrated toward cooler temperatures. At placement temperatures of 20 degrees C or below, few or no larvae moved toward the zone of higher temperature. Larvae isolated from rat feces cultured at 20 degrees C tended to migrate to a high temperature area regardless of placed temperature. Those cultured at 30 degrees C did not respond to the temperature gradient. L3 cultured at 30 degrees C were significantly less infective to rats than those cultured at 25 degrees or 20 degrees C. Additional experiments were designed to demonstrate thermokinetic behaviors during the period after reaching the L3 stage. Larvae incubated in double distilled water (DDW) for 24 h at 37 degrees C lost their ability to respond to lower temperatures, while in those incubated in DDW at 15 degrees and 25 degrees C, responses were still apparent. The thermokinetic behavior of S. ratti L3 is affected by surrounding environmental temperatures and this may have an important role in host finding.