wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila
wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila
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DOI：
10.1016/s0896-6273(02)00589-5
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发表时间：
2002-02-14
期刊：
影响因子：
16.2
通讯作者：
Goodman, CS
中科院分区：
文献类型：
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作者：
Aberle, H;Haghighi, AP;Goodman, CS
我们进行了一个大规模的屏幕果蝇突变体的幼虫神经肌肉接头（NMJ）的结构异常。我们恢复了一厢情愿（wish thinking，wit）的突变，wit是一种积极调节突触生长的基因。Wit编码BMP II型受体。在WIT突变的幼虫中，NMJ的大小相对于肌肉的大小大大减少。其中NMJ具有降低的诱发兴奋性连接电位、降低的突触细胞粘附分子Fasciclin II水平和活动区处的突触膜脱离。机智是由一部分神经元表达的，包括运动神经元。NMJ表型通过仅在运动神经元中转基因表达Wit而被特异性拯救。因此，机智似乎作为突触前受体，调节突触的大小在果蝇NMJ。
We conducted a large-scale screen for Drosophila mutants that have structural abnormalities of the larval neuromuscular junction (NMJ). We recovered mutations in wishful thinking (wit), a gene that positively regulates synaptic growth. wit encodes a BMP type II receptor. In wit mutant larvae, the size of the NMJs is greatly reduced relative to the size of the muscles. wit NMJs have reduced evoked excitatory junctional potentials, decreased levels of the synaptic cell adhesion molecule Fasciclin II, and synaptic membrane detachment at active zones. Wit is expressed by a subset of neurons, including motoneurons. The NMJ phenotype is specifically rescued by transgenic expression of Wit only in motoneurons. Thus, Wit appears to function as a presynaptic receptor that regulates synaptic size at the Drosophila NMJ.