Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp in apple seedlings
Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp in apple seedlings
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DOI：
10.1007/s13213-013-0672-3
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发表时间：
2014-03-01
影响因子：
3
通讯作者：
Liu, Xunli
中科院分区：
文献类型：
--
作者：
Ju, Ruicheng;Zhao, Yuhua;Liu, Xunli
为寻找防治苹果枯萎病的有效生防菌，从苹果树体组织中分离到一株内生细菌。测定了该菌株对镰刀菌和立枯丝核菌菌丝生长的抑制作用。菌株Y-1对黄瓜枯萎病菌、黄瓜moniliforme、串珠镰刀菌F. proliferatum，F. solani和R.索拉尼其对F.尖孢霉（oxysporum）最高，达64.90%。活体试验表明，Y-1菌株对苹果的抗赤霉病有较好的保护作用。尖孢菌感染。接种前3d进行细菌接种，防效达92.26%.菌株Y-1在30 d内即可在根际和组织中定殖。SOD、POD活性测定结果表明，该处理能诱导苹果幼苗产生系统抗性。与对照组相比，菌株Y-1显著增加了苹果幼苗的根长、根干重和根湿重以及株高。16S rRNA序列同源性分析以及形态、理化和生化分析表明，菌株Y-1为枯草芽孢杆菌（Bacillus subtilis）。
To find a potential biocontrol agent against Fusarium sp. in apple seedlings, an endophytic bacterium strain was isolated from apple tree tissues. The inhibitive efficiency of the isolated strain against the hyphal growth of Fusarium sp. and Rhizoctonia solani was tested. Strain Y-1 showed significant inhibitory effects against Fusarium oxysporum, F. moniliforme, F. proliferatum, F. solani and R. solani. Its antifungal activity against F. oxysporum was the highest, reaching up to 64.90 %. In vivo tests indicated that strain Y-1 effectively protects apple from F. oxysporum infections. The control effect reached 92.26 % when bacterial inoculation was performed 3 days prior to pathogen inoculation. Strain Y-1 could colonize the rhizosphere and tissues within 30 days. It was also able to induce systemic resistance in apple seedlings as shown by the activities of SOD and POD. Strain Y-1 significantly increased the root length, root wet and dry weights, and plant height of the apple seedlings compared with the control group. The homology analysis of the 16S rRNA sequence, together with morphological, physical, and biochemical analyses, revealed that strain Y-1 is Bacillus subtilis.