Depth-resolved image mapping spectrometer (IMS) with structured illumination.
Depth-resolved image mapping spectrometer (IMS) with structured illumination.
复制标题
深度分辨图像映射光谱仪（IMS）带有结构化照明。
DOI：
10.1364/oe.19.017439
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发表时间：
2011-08-29
期刊：
影响因子：
3.8
通讯作者：
Tkaczyk TS
中科院分区：
文献类型：
--
作者：
Gao L;Bedard N;Hagen N;Kester RT;Tkaczyk TS
本文介绍了一种能够获取4D（x，y，z，λ）数据立方体的深度分辨成像光谱仪（IMS）。光学切片通过结构化照明来实现。在多光谱微球和小鼠肾脏组织荧光成像实验中证明了该器件的光谱成像性能。我们还比较定量的深度分辨IMS与高光谱共聚焦显微镜（HCM）在一个标准的荧光珠成像实验。比较结果表明，尽管在IMS中使用的光源强度比HCM低四个数量级，但IMS获得的图像信噪比比等效共焦方法高2.6倍。
We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device’s spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.