DNA-SEQUENCE FOR CLONED CDNA FOR MURINE AMELOGENIN REVEAL THE AMINO-ACID SEQUENCE FOR ENAMEL-SPECIFIC PROTEIN
DNA-SEQUENCE FOR CLONED CDNA FOR MURINE AMELOGENIN REVEAL THE AMINO-ACID SEQUENCE FOR ENAMEL-SPECIFIC PROTEIN
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DOI：
10.1016/0006-291x(85)91964-3
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
SLAVKIN, HC
中科院分区：
文献类型：
--
作者：
SNEAD, ML;LAU, EC;SLAVKIN, HC
牙釉质是覆盖脊椎动物牙齿的独特的高度矿化的细胞外基质。釉原蛋白是哺乳动物牙釉质发育过程中基因产物的主要亚家族，参与调节脊椎动物体内最大羟基磷灰石晶体的形成。以前的尝试，分离，纯化和表征从显影基质中提取的釉原蛋白已被证明是困难的。26-kDa [千道尔顿]类鼠釉原蛋白的[互补] cDNA的DNA序列，并推导出其相应的氨基酸序列。鼠氨基酸序列与从发育中的釉质基质中提取的牛或猪釉质原蛋白同源。在鼠釉原蛋白的羧基末端发现另外的10个残基。这是最完整的釉原蛋白肽序列数据库，也是釉特异性基因的唯一DNA序列。
Enamel is the unique and highly mineralized extracellular matrix that covers vertebrate teeth. Amelogenin proteins represent the predominate subfamily of gene products found in developing mammalian enamel, and are implicated in the regulation of the formation of the largest hydroxyapatite crystals in the vertebrate body. Previous attempts to isolate, purify and characterize amelogenins extracted from developing matrix have proven difficult. The DNA sequence for a [complementary] cDNA for the 26-kDa [kilodalton] class of murine amelogenin and deduced its corresponding amino acid sequence. The murine amino acid sequence is homologous to bovine or porcine amelogenins extracted from developing enamel matrices. An additional 10-residues were found at the carboxy terminus of the murine amelogenin. This is the most complete sequence database for amelogenin peptides and the only DNA sequence for enamel specific genes.