Ecotoxicological effects of the herbicide glyphosate in non-target aquatic species: Transcriptional responses in the mussel Mytilus galloprovincialis.
Ecotoxicological effects of the herbicide glyphosate in non-target aquatic species: Transcriptional responses in the mussel Mytilus galloprovincialis.
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DOI：
10.1016/j.envpol.2018.02.049
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发表时间：
2018-06
影响因子：
8.9
通讯作者：
M. Milan;G. Rovere;M. Smits;S. Ferraresso;P. Pastore;M. Marin;S. Bogialli;T. Patarnello;L. Bargelloni;V. Matozzo
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文献类型：
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作者：
M. Milan;G. Rovere;M. Smits;S. Ferraresso;P. Pastore;M. Marin;S. Bogialli;T. Patarnello;L. Bargelloni;V. Matozzo
在过去的三十年里，草甘膦一直是世界上使用最广泛的除草剂，其对环境和人类健康的潜在影响引起了越来越多的关注。最近的研究表明，草甘膦存在于土壤、地表水和地下水中，残留物存在于食物链的各个层面，如饮用水、植物、动物，甚至人类体内。虽然研究表明草甘膦可以在暴露的生物体中引起广泛的生物效应，但仍需要阐明全球分子作用机制，特别是对海洋物种。在这项研究中，我们的特点是第一次草甘膦在海洋双壳类动物暴露于环境现实浓度后的分子作用机制。为了实现这一目标，地中海贻贝Mytilus galloprovincialis，一个生态和经济上相关的物种，暴露于10，100和1000 μ g/L 21天，并通过RNA-seq研究消化腺转录谱。差异表达分析分别鉴定了草甘膦浓度为10、100和1000 μ g/L时总共111、124和211个差异调节的转录物。在所有研究的浓度下，发现五个基因一致差异表达，包括SERP2，其在保护未折叠靶蛋白免受降解中起作用，抗凋亡蛋白GIMAP5和MTMR14，其参与大自噬。差异表达基因的功能分析揭示了几个关键的生物过程，如能量代谢和Ca2+稳态，细胞信号传导和内质网应激反应的中断。总之，所获得的结果表明，草甘膦在海洋生态系统中的存在应引起特别关注，因为即使在最低浓度下也会产生重大影响。
Glyphosate has been the most widely used herbicide worldwide over the last three decades, raising increasing concerns for its potential impacts on environmental and human health. Recent studies revealed that glyphosate occurs in soil, surface water, and groundwater, and residues are found at all levels of the food chain, such as drinking water, plants, animals, and even in humans. While research has demonstrated that glyphosate can induce a broad range of biological effects in exposed organisms, the global molecular mechanisms of action still need to be elucidated, in particular for marine species. In this study, we characterized for the first time the molecular mechanisms of action of glyphosate in a marine bivalve species after exposure to environmentally realistic concentrations. To reach such a goal, Mediterranean musselsMytilus galloprovincialis, an ecologically and economically relevant species, were exposed for 21 days to 10, 100, and 1000 μg/L and digestive gland transcriptional profiles were investigated through RNA-seq. Differential expression analysis identified a total of 111, 124, and 211 differentially regulated transcripts at glyphosate concentrations of 10, 100, and 1000 μg/L, respectively. Five genes were found consistently differentially expressed at all investigated concentrations, including SERP2, which plays a role in the protection of unfolded target proteins against degradation, the antiapoptotic protein GIMAP5, and MTMR14, which is involved in macroautophagy. Functional analysis of differentially expressed genes reveals the disruption of several key biological processes, such as energy metabolism and Ca2+homeostasis, cell signalling, and endoplasmic reticulum stress response. Together, the results obtained suggest that the presence of glyphosate in the marine ecosystem should raise particular concern because of its significant effects even at the lowest concentration.