Metal distribution and metallothionein in loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtles
Metal distribution and metallothionein in loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtles
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DOI：
10.1016/j.scitotenv.2007.09.014
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发表时间：
2008-02-01
影响因子：
9.8
通讯作者：
Isani, Gloria
中科院分区：
文献类型：
--
作者：
Andreani, Giulia;Santoro, Mario;Isani, Gloria
我们研究的第一个目的是确定选定的微量元素（锌，铜，铁，锰，镉和铅）的组织中的绿色海龟从托尔图格罗国家公园在北加勒比海沿岸的哥斯达黎加和红海龟从地中海的浓度。Zn，Cu，Fe，Mn和Cd在所有样品中均以可检测浓度存在，并表现出明显的有机性，而Pb并不总是超过检测限，也没有表现出任何特定的组织分布。这两个物种呈现出显着差异：Cu和Cd在Chelonia mydas的肝脏和肾脏中的浓度显着高于Caretta caretta.我们的研究的第二个和主要目标是评估肝脏和肾脏金属硫蛋白（MT）作为环境金属暴露的生物标志物。本文首次对中华鳖肾脏、肝脏和绿色鳖肾脏中的MT进行了定量研究。MT浓度在绿色比红海龟。此外，两种鱼肝脏和肾脏中Cu和Cd含量与Cu-MT和Cd-MT呈正相关，提示MT在金属储存和解毒中起着关键作用。只要采用准确的分析方法，肝脏和肾脏中金属和MT的定量分析可以作为水环境中金属暴露的有效生物标志物，用于评估海龟的健康状况。(c)2007 Elsevier B. V.保留所有权利。
The first aim of our study was to determine the concentrations of selected trace elements (Zn, Cu, Fe, Mn, Cd and Pb) in tissues of green turtles from Tortuguero National Park on the North Caribbean coast of Costa Rica and of loggerheads from the Mediterranean Sea. Zn, Cu, Fe, Mn and Cd were present at detectable concentrations in all samples and showed clear organotropism, whereas Pb was not always over the detection limit and did not show any particular tissue distribution. The two species presented significant differences: Cu and Cd in liver and kidney of Chelonia mydas were significantly higher with respect to the concentrations found in Caretta caretta.The second and major goal of our study was to evaluate hepatic and renal metallothionein (MT) as a biomarker of environmental metal exposure. The present paper is the first to describe and quantify MT in kidney and liver of loggerhead turtles and in kidney of green turtles. MT concentrations were higher in green than in loggerhead turtles. In addition, positive correlations were found between Cu and Cd concentrations and Cu-MT and Cd-MT in liver and kidney in both species, suggesting a pivotal role of MT in metal storage and detoxification. The quantification of metals and MT in liver and kidney may be a valid biomarker of metal exposure in the aquatic environment to assess the health of marine sea turtles as long as accurate analytical methods are adopted. (c) 2007 Elsevier B.V. All rights reserved.