The Effect of the Addition of Adipose-Derived Mesenchymal Stem Cells to a Meniscal Repair in the Avascular Zone: An Experimental Study in Rabbits
The Effect of the Addition of Adipose-Derived Mesenchymal Stem Cells to a Meniscal Repair in the Avascular Zone: An Experimental Study in Rabbits
复制标题
DOI：
10.1016/j.arthro.2011.06.041
复制
发表时间：
2011-12-01
影响因子：
4.7
通讯作者：
Abraira, Victor
中科院分区：
文献类型：
--
作者：
Angel Ruiz-Iban, Miguel;Diaz-Heredia, Jorge;Abraira, Victor
目的：目的：探讨脂肪间充质干细胞（ASC）对兔无血管区创面愈合的影响。方法：采用四组实验。在A组（n = 12）中，在内侧半月板前角的无血管区创建一个短的、5 mm长的纵向损伤，并立即缝合。B组（n = 8）也制作同样的短病变，但延迟3周缝合。C组（n = 12），创建一个较大的，15 mm长的病变，横跨整个半月板，并立即缝合。D组（n = 8）3周后缝合同一大病灶。使用每只家兔的两个膝关节：1个作为对照，另一个在即将植入前将1 x 10（5）个溴脱氧尿苷标记的同种异体ASC置于病变中。在12周时处死动物。结果：在A组（短病变，急性修复）中，6/12例ASC治疗组和0/12例对照组有一定程度的愈合（P = 0.014）。在B组（短病变，延迟修复）中，2/8例ASC治疗组和1/8例对照组有一定程度的愈合（P = 0.5）。在C组（长病变，急性修复）中，6/12例ASC治疗组和0/12例对照组有一定程度的愈合（P = 0.014）。在D组（长病变，延迟修复）中，4/8例ASC治疗组和0/8例对照组有一定程度的愈合（P = 0.07）。添加ASC增加了愈合率（比值比，32 [范围，3.69至277]; P = .002）。愈合区的组织学分析鉴定出具有源自ASC的持续存在的细胞的形成良好的椎间盘纤维软骨（用抗溴脱氧尿苷抗体免疫定位）。结论：在兔髂动脉无血管区的修复中加入ASCs可以增加愈合的机会。在较小和较大的病变中，愈合得到改善。当缝合延迟时，效果不明显。临床意义：将来，ASCs可能有助于无血管区的血管修复。
Purpose: To determine whether adipose-derived mesenchymal stem cells (ASCs) affect the healing rate of meniscal lesions sutured in the avascular zone in rabbits. Methods: Four groups were used. In group A (n = 12) a short, 5-mm-long longitudinal lesion in the avascular zone of the anterior horn of the medial meniscus was created and immediately sutured. In group B (n = 8) the same short lesion was created but suture was delayed 3 weeks. In group C (n = 12) a larger, 15-mm-long lesion that spanned the whole meniscus was created and sutured immediately. In group D (n = 8) the same large lesion was sutured 3 weeks later. Both knees in each rabbit were used: 1 served as the control, and in the other, 1 x 10(5) allogeneic ASCs marked with bromodeoxyuridine were placed in the lesion immediately before suturing. The animals were killed at 12 weeks. Results: In group A (short lesion, acute repair) 6 of 12 ASC-treated menisci and 0 of 12 controls had some healing (P = .014). In group B (short lesion, delayed repair) 2 of 8 ASC-treated menisci and 1 of 8 controls had some healing (P = .5). In group C (long lesion, acute repair) 6 of 12 ASC-treated menisci and 0 of 12 controls had some healing (P = .014). In group D (long lesion, delayed repair) 4 of 8 ASC-treated menisci and 0 of 8 controls had some healing (P = .07). The addition of ASCs increased the healing rate (odds ratio, 32 [range, 3.69 to 277]; P = .002). The histologic analysis of the healed zones identified well-formed meniscal fibrocartilage with persistence of cells derived from the ASCs (immunolocated with anti-bromodeoxyuridine antibodies). Conclusions: Adding ASCs to a repair in the avascular zone of rabbit menisci increases the chances of healing. Healing is improved in small and larger lesions. When suture is delayed, the effect is not as evident. Clinical Relevance: In the future, ASCs might help in meniscal repair in the avascular zone.