Two-Stage Dynamics of In Vivo Bacteriophage Genome Ejection
Two-Stage Dynamics of In Vivo Bacteriophage Genome Ejection
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DOI：
10.1103/physrevx.8.021029
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发表时间：
2018-05-01
影响因子：
12.5
通讯作者：
Kegel, Willem K.
中科院分区：
文献类型：
--
作者：
Chen, Yi-Ju;Wu, David;Kegel, Willem K.
生物聚合物转运是病毒感染过程中的关键步骤。编码信息的基因组的转移使病毒能够重新编程它们宿主的细胞命运。占所有已知细菌病毒的96%[A.Fokine和M.G.Rossmann，MolectoryofTailingDoustredDNAphages，phaciphage 4，e28281(2014)]，尾部噬菌体通过“喷射”过程将它们的DNA输送到宿主细胞中，将它们的蛋白质外壳留在细菌之外；类似的情况发生在哺乳动物病毒中，DNA基因组被喷射到宿主细胞的细胞核中，而病毒衣壳仍然结合在核孔复合体的外面。根据以前对体内噬菌体的实验测量。抛射，我们在这里分析引起观测到的动力学的物理过程。我们认为，在衣壳中的DNA自排斥驱动的初始阶段之后，抛射是由拥挤的细菌细胞质中噬菌体DNA的异常扩散驱动的。我们预计，这种两步机制对通过压力驱动喷射操作的噬菌体是通用的，并讨论了我们的理论的预测，将在未来的实验中得到验证。
Biopolymer translocation is a key step in viral infection processes. The transfer of information-encoding genomes allows viruses to reprogram the cell fate of their hosts. Constituting 96% of all known bacterial viruses [A. Fokine and M. G. Rossmann, Molecular architecture of tailed double-stranded DNA phages, Bacteriophage 4, e28281 (2014)], the tailed bacteriophages deliver their DNA into host cells via an "ejection" process, leaving their protein shells outside of the bacteria; a similar scenario occurs for mammalian viruses like herpes, where the DNA genome is ejected into the nucleus of host cells, while the viral capsid remains bound outside to a nuclear-pore complex. In light of previous experimental measurements of in vivo bacteriophage. ejection, we analyze here the physical processes that give rise to the observed dynamics. We propose that, after an initial phase driven by self-repulsion of DNA in the capsid, the ejection is driven by anomalous diffusion of phage DNA in the crowded bacterial cytoplasm. We expect that this two-step mechanism is general for phages that operate by pressure-driven ejection, and we discuss predictions of our theory to be tested in future experiments.