Evolutionary developmental transcriptomics reveals a gene network module regulating interspecific diversity in plant leaf shape
Evolutionary developmental transcriptomics reveals a gene network module regulating interspecific diversity in plant leaf shape
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DOI：
10.1073/pnas.1402835111
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发表时间：
2014-06-24
影响因子：
11.1
通讯作者：
Sinha, Neelima R.
中科院分区：
文献类型：
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作者：
Ichihashi, Yasunori;Aguilar-Martinez, Jose Antonio;Sinha, Neelima R.
尽管长期以来对形态多样性的遗传基础感兴趣，但引起发育变异的分子机制尚未完全理解。在这里，我们使用比较转录组学加上基因共表达网络的构建来预测番茄和两个相关的野生物种的叶片发育的基因调控网络（GRN），具有显着不同的叶形态。叶发育GRN中的核心网络包含叶形态的调节因子，其与外周基因网络模块（GNM）一起在全局细胞增殖中起作用。一个GNM中的叶柄上叶片（BOP）转录因子通过改变结样HOMEOBOX基因产物的有效浓度来控制核心网络。BOP水平的比较网络分析和实验扰动表明，BOP表达的变化可以解释这些物种之间的叶复杂性的多样性，通过动态重新布线的GRN的相互作用。在叶发育GRN和BOP水平的改变所引起的进化多样性的表型模拟的BOP-含有GNM的外围位置确定了一个关键作用，这个GNM在通过修改叶片的成熟时间表，以创建形态多样性的叶形态空间的渠道。
Despite a long-standing interest in the genetic basis of morphological diversity, the molecular mechanisms that give rise to developmental variation are incompletely understood. Here, we use comparative transcriptomics coupled with the construction of gene coexpression networks to predict a gene regulatory network (GRN) for leaf development in tomato and two related wild species with strikingly different leaf morphologies. The core network in the leaf developmental GRN contains regulators of leaf morphology that function in global cell proliferation with peripheral gene network modules (GNMs). The BLADE-ON-PETIOLE (BOP) transcription factor in one GNM controls the core network by altering effective concentration of the KNOTTED-like HOMEOBOX gene product. Comparative network analysis and experimental perturbations of BOP levels suggest that variation in BOP expression could explain the diversity in leaf complexity among these species through dynamic rewiring of interactions in the GRN. The peripheral location of the BOP-containing GNM in the leaf developmental GRN and the phenotypic mimics of evolutionary diversity caused by alteration in BOP levels identify a key role for this GNM in canalizing the leaf morphospace by modifying the maturation schedule of leaves to create morphological diversity.