Sperm surface protein profiles of fertile and infertile men: search for a diagnostic molecular marker.
Sperm surface protein profiles of fertile and infertile men: search for a diagnostic molecular marker.
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生育和不育男性的精子表面蛋白谱：寻找诊断分子标记。
DOI：
10.1080/014850199262463
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Leslie,MH
中科院分区：
文献类型：
--
作者：
Naz,RK;Leslie,MH
本研究的目的是检查可育男性（n = 37）和不育男性（n = 29）的精子膜蛋白谱是否存在差异。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）中，可育男性和不育男性精子提取物在不同分子区域显示出12-16个主要和8-14个次要的共同蛋白带。然而，在约80%不育男性的精子提取物中存在约78kD的条带，而在可育男性的精子提取物中不存在。在其余约20%的不育男性中，其强度相对较弱。该条带仅存在于精子提取物中，而不存在于精浆中，无论是可育男性还是不育男性，并被指定为不育相关精子蛋白（IASP）。IASP抗体特异性地免疫沉淀了不育和非可育男性精子提取物中的~78-kD蛋白。在免疫荧光技术中，抗iasp抗体与形态异常精子的头部和/或中部区域发生强烈反应，并与可育男性形态正常精子的赤道区域的蛋白质发生交叉反应。IASP分子及其抗体可能提供区分正常和异常精子的分子工具，因此可能是诊断男性因素不育的有用标记。
The present study was conducted to examine whether there is any difference in the protein profiles of sperm membranes of fertile (n = 37) and infertile (n = 29) men. In the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) procedure, the sperm extracts of fertile and infertile men showed 12-16 major and 8-14 minor common protein bands in various molecular regions. However, there was an additional band of ~78kD present in the sperm extracts of ~80% of infertile men that was absent in the sperm extracts of fertile men. In the remaining ~20% of the infertile men, it was of relatively weaker intensity. This band was present only in the sperm extracts and not in the seminal plasma, whether of fertile or infertile men, and was designated as the infertility-associated sperm protein (IASP).Antibodies to IASP specifically immunoprecipitated the ~78-kD protein from the sperm extracts of only infertile and not the fertile men. In the immunofluorescence technique, the anti-IASP antibodies reacted strongly with the head and/or midpiece regions of morphologically abnormal sperm and cross-reacted with a protein in the equatorial regions of morphologically normal sperm of fertile men. The IASP molecule and its antibodies may provide molecular tools to differentiate between the normal and abnormal sperm, and, thus, may be useful markers for the diagnosis of male-factor infertility.