STUDIES ON THE IDENTIFICATION OF CONSTITUENTS IN ETHANOL EXTRACT OF RADIX GLYCYRRHIZAE AND THEIR ANTICANCER ACTIVITY
STUDIES ON THE IDENTIFICATION OF CONSTITUENTS IN ETHANOL EXTRACT OF RADIX GLYCYRRHIZAE AND THEIR ANTICANCER ACTIVITY
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DOI：
10.4314/ajtcam.v11i2.18
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Li, Xu
中科院分区：
文献类型：
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作者：
Shi, Xiaoli;Zou, Ming;Li, Xu
背景资料：黄芪是一种临床应用广泛的传统中药，具有和中、润肺、解毒、调和诸药的功效。为分离鉴定川牛膝乙醇提取物中的化学成分，并研究其抗肿瘤活性，采用柱层析、ODS柱层析、制备薄层层析和核磁共振波谱技术对川牛膝乙醇提取物中的化学成分进行分离鉴定;采用光学显微镜和流式细胞仪检测葛根提取物的抗肿瘤作用。从甘草乙醇提取物中分离得到4个化合物，分别为齐墩果酸、异甘草苷、甘草次酸和甘草查尔酮A。光学显微镜观察发现，各实验组胃癌细胞株SGC-7901生长受到抑制，贴壁细胞出现凋亡形态学改变; PI染色流式细胞仪检测结果显示，与对照组相比，板蓝根提取物在0.5- 1.5mg/mL浓度范围内可诱导SGC-7901细胞凋亡，结论：板蓝根醇提物对胃癌细胞株SGC-7901具有一定的抑制增殖作用。
Background: The main functions of Radix Glycyrrhizae include regulating middle warmer, moistening lung, relieving toxicity, harmonizing property of drugs which is a traditional Chinese medicine widely used in clinical settings. The objective of the paper is to isolate and identify the constituents in ethanol extract of Radix Glycyrrhizae, and to study their anticancer activity.Materials and Methods: Column chromatography, ODS column chromatography, preparative thin layer chromatography and NMR spectroscopy techniques were used to isolate compounds from ethanol extract of Radix Glycyrrhizae; optical microscopy and flow cytometry were used to determine the anticancer effect of Radix Glycyrrhizae extract.Results: Four compounds were isolated from the ethanol extract of Radix Glycyrrhizae, namely oleanolic acid, isoliquiritin, glycyrrhetinic acid and licochalcone A. Optical microscopic observation showed that the growth of gastric cancer cell line SGC-7901 was inhibited in the experimental groups, and apoptotic morphological changes were observed in adherent cells; flow cytometry with PI staining showed that Radix Glycyrrhizae extract could induce SGC-7901 cell apoptosis within a concentration range of 0.5-1.5 mg/mL, compared with the control group, the apoptosis was positively correlated with the drug concentration, which exhibited an apparent dose-dependence.Conclusion: We conclude Ethanol extract of Radix Glycyrrhizae has an anti-proliferative activity on SGC-7901 cells.