Remote in vivo imaging of human skin corneocytes by means of an optical fiber bundle
Remote in vivo imaging of human skin corneocytes by means of an optical fiber bundle
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DOI：
10.1063/1.2736346
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发表时间：
2007-05-01
影响因子：
1.6
通讯作者：
Sojic, Neso
中科院分区：
文献类型：
--
作者：
Dromard, Tanguy;Ravaine, Valerie;Sojic, Neso
形成表皮最外层（角质层）的人角质细胞通过相干光纤束通过落射荧光在体内成像。提出了一种非常简单和快速的方法来远程可视化形成皮肤保护层的细胞。在局部应用荧光素后，将光纤束的远端面轻轻地垂直应用到标记的皮肤上（接触模式）。通过包括30000个单独包覆的3.5 μ m直径光纤的束，在50 ms内获得角质细胞的远程荧光图像。非常短的焦距是这种束的固有特征，仅允许可视化与外部环境接触的最表面的单层细胞。一幅图像直接显示人体上大约400 - 500个细胞。因此，可以以非常简单和容易的方式获得和分析角质细胞的大小和排列。该方法灵活，可用于人体的任何部位。使用融合到束的远侧面的梯度折射率透镜物镜（放大率2.8倍）可以更好地分辨角质细胞的形状。此外，工作距离为300 μ m，因此该第二种方法以非接触成像模式工作。这两种方法是互补的，并且允许即时提供细胞的全局视图，其具有可能的区域或形态信息的统计确定，这对于皮肤病学和美容科学是必不可少的。最后，为了提高记录图像的质量和对比度，我们测试了含有荧光素的二氧化硅纳米颗粒。简而言之，这种诊断方法是无毒，无痛，易于使用，非侵入性和非破坏性的。(C)2007年，美国物理学会。
Human corneocytes forming the outermost layer of the epidermis (stratum corneum) were imaged in vivo by epifluorescence through a coherent optical fiber bundle. A very simple and rapid method to remotely visualize the cells forming this protective layer of the skin is presented. After the topical application of fluorescein, the distal face of an optical fiber bundle is gently applied perpendicularly onto the labeled skin (contact mode). Remote fluorescence images of the corneocytes are acquired in 50 ms through the bundle comprising 30 000 individually cladded 3.5 mu m diameter optical fibers. The very short focal distance which is an intrinsic characteristic of such bundles, allows visualizing only the most superficial monolayer of cells in contact with the external environment. An image displays about 400-500 cells directly on the human body. The size and the arrangement of the corneocytes can thus be acquired and analyzed in a very simple and easy way. The method is flexible and can be used for any location on the human body. Using a gradient-index lens objective (magnification 2.8x) fused to the distal face of the bundle allows the shape of the corneocytes to be better resolved. In addition, the working distance is 300 mu m and hence this second approach works in a noncontact imaging mode. Both approaches are complementary and allow providing instantaneously either a global view of the cells with a possible statistical determination of their area or morphological information, which are essential for dermatology and cosmetic sciences. Finally, to improve the quality and the contrast of the recorded images, we tested silica nanoparticles containing fluorescein. In brief, this diagnostic method is nontoxic, painless, easy to use, noninvasive, and nondestructive. (C) 2007 American Institute of Physics.