Cell-Cycle Analysis in 20 Minutes
Cell-Cycle Analysis in 20 Minutes
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20 分钟内完成细胞周期分析
作者：
H. Crissman;R. Tobey
将光神霉素加入到固定在含水乙醇中的哺乳动物细胞中，与DNA结合，并与细胞DNA含量成正比地发出荧光。通过高速流动系统仪器的定量荧光测量提供了一种快速的细胞周期分析方法，并首次允许在正在进行的实验中连续监测细胞周期动力学。
Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.