Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method.
Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method.
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用弯曲杆菌可靠地定植肉鸡。
DOI：
10.1080/00071668.2018.1523538
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发表时间：
2018
影响因子：
2
通讯作者：
Sandilands V
中科院分区：
文献类型：
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作者：
Sandilands V
1.鸡相关弯曲杆菌。是欧洲大多数食物中毒病例的起因。为了研究宿主与病原体的相互作用，需要一种可靠和可重复性的方法来定植带有细菌的鸡。这项研究的目的是确定一种更合适、侵入性更小的殖民方法(参看。用弯曲杆菌、肉汤和粪便的混合物饲养商品鸡的垫料(窝窝)。研究的第一阶段测试了弯曲杆菌的寿命。在24小时以上的种子凋落物中恢复：显著高于弯曲杆菌。病原菌在播种后0或3小时恢复，而不是播后6小时和24小时，这表明病原菌在这种环境中可以存活到可检测到的水平至少3小时。在第二阶段，三组10只肉鸡(弯曲杆菌阴性)。在暴露之前)在21天龄时暴露于三种不同的空肠弯曲菌中的一种。(A，B，C)，使用上述方法。在28天龄时，通过过量注射巴比妥酸盐或颈椎脱位对禽类实施安乐死，并切除肝脏和盲肠以供弯曲菌使用。评估。所有肝脏和28/30份盲肠标本均为弯曲杆菌阳性，其中A和C混合物盲肠中的细菌计数高于B混合物。安乐死方法对弯曲杆菌没有影响。数一数。总之，弯曲杆菌是一种可靠地定植肉鸡的成功方法。已经开发出来，它不需要灌胃，更能代表现场污染是如何发生的。
1. Chicken-associatedCampylobacterspp. are the cause of most food poisoning cases in Europe. In order to study the host–pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required.2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture ofCampylobacterspp., broth and faeces.3. The first phase of the study tested the longevity ofCampylobacterspp. recovery in seeded litter over 24 h: significantly moreCampylobacterspp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment.4. In the second phase, three groups of 10 broiler chickens (negative forCampylobacterspp. prior to exposure) were exposed at 21 days of age to one of three differentCampylobacter jejuniandC. colimixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed forCampylobacterspp. assessment.5. All liver and 28/30 caeca samples tested positive forCampylobacterspp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affectCampylobacterspp. counts.6. In conclusion, a successful method for reliably colonising broiler chickens withCampylobacterspp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.