Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity.
Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity.
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DOI：
10.3389/fcvm.2021.631650
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发表时间：
2021
影响因子：
3.6
通讯作者：
Peng J
中科院分区：
文献类型：
--
作者：
Chen N;Miao L;Lin W;Zou D;Huang L;Huang J;Shi W;Li L;Luo Y;Liang H;Pan S;Peng J
背景：探讨DNA甲基化与基因表达在肥胖病理中的关系。研究方法：（1）建立肥胖和正常人内脏脂肪组织（VAT）基因表达谱数据库，分析VAT基因组DNA甲基化和mRNA表达谱。(2)进行功能富集分析和差异甲基化基因调控网络的构建。(3)在单独的微阵列数据集中进行两个不同甲基化位点和相应基因表达的验证。(4)对DNA甲基化和mRNA表达数据进行相关性分析。结果：共筛选出77条差异表达的mRNA与差异甲基化基因相匹配。分析发现两个不同的甲基化位点对应于两个独特的基因s100 a8-cg 09174555和s100 a9-cg 03165378。通过对两个有趣的不同表达位置[差异甲基化位置（DMPs）]及其相应基因表达的验证性检验，发现肥胖组中这些基因的甲基化与基因表达呈负相关。在单独的微阵列数据集中验证了肥胖受试者中较高的S100 A8和S100 A9表达。结论：本研究证实了DNA甲基化与基因表达的关系，强调了S100 A8和S100 A9在肥胖发病中的重要作用。
Background: To explore the association of DNA methylation and gene expression in the pathology of obesity. Methods: (1) Genomic DNA methylation and mRNA expression profile of visceral adipose tissue (VAT) were performed in a comprehensive database of gene expression in obese and normal subjects. (2) Functional enrichment analysis and construction of differential methylation gene regulatory networks were performed. (3) Validation of the two different methylation sites and corresponding gene expression was done in a separate microarray dataset. (4) Correlation analysis was performed on DNA methylation and mRNA expression data. Results: A total of 77 differentially expressed mRNAs matched with differentially methylated genes. Analysis revealed two different methylation sites corresponding to two unique genes—s100a8-cg09174555 and s100a9-cg03165378. Through the verification test of two interesting different expression positions [differentially methylated positions (DMPs)] and their corresponding gene expression, we found that methylation in these genes was negatively correlated to gene expression in the obesity group. Higher S100A8 and S100A9 expressions in obese subjects were validated in a separate microarray dataset. Conclusion: This study confirmed the relationship between DNA methylation and gene expression and emphasized the important role of S100A8 and S100A9 in the pathogenesis of obesity.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.2
作者：
Miao, Liu;Yin, Rui-Xing;Wu, Jin-Zhen
通讯作者：
Wu, Jin-Zhen
影响因子：
168.9
作者：
Dick, Katherine J.;Nelson, Christopher P.;Samani, Nilesh J.
通讯作者：
Samani, Nilesh J.
影响因子：
7.4
作者：
Miao, Liu;Yin, Rui-Xing;Lin, Wei-Xiong
通讯作者：
Lin, Wei-Xiong
影响因子：
3
作者：
Bader, GD;Hogue, CW
通讯作者：
Hogue, CW