Testosterone and estradiol up-regulate androgen and estrogen receptors in immature and adult rat thyroid glands in vivo
Testosterone and estradiol up-regulate androgen and estrogen receptors in immature and adult rat thyroid glands in vivo
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DOI：
10.1016/s0039-128x(02)00063-6
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发表时间：
2002-12-01
期刊：
影响因子：
2.7
通讯作者：
Aruldhas, MM
中科院分区：
文献类型：
--
作者：
Banu, SK;Govindarajulu, P;Aruldhas, MM
甲状腺是性类固醇激素的非经典靶器官之一。雄激素和雌激素受体存在于肿瘤和非肿瘤性甲状腺的动物物种是有据可查的。本研究的目的是阐明在性腺切除（GDX）和性类固醇替代条件下，从未成熟到成年大鼠血清和甲状腺性类固醇及其受体的变化。从未成熟到成年的正常Wistar雄性和雌性大鼠（第21、30、45天）。60和160产后（pp））在本研究中使用。一组（I）大鼠在早期（第1天Opp）时为GDX，另一组（II）在成年（第120天pp）时。I组大鼠于产后21、30、45、60 d处死，II组大鼠于产后160 d处死。另一组GDX大鼠从组I和II被替换为生理剂量的睾酮或雌二醇。用放射免疫分析法测定血清和甲状腺的性激素浓度，用放射受体分析法测定受体浓度。性腺切除术显着降低血清和甲状腺睾酮和雌二醇和雄激素受体（AR）和雌激素受体（ER）在甲状腺中的浓度。GDX大鼠经性激素替代治疗后，性激素、AR和ER水平恢复正常。因此，本研究表明：（i）性类固醇上调其自身在甲状腺中的受体，（ii）性类固醇可能通过调节其在甲状腺中的受体浓度来影响甲状腺生长和甲状腺细胞增殖。(C)2002年由Elsevier Science Inc.出版
Thyroid gland is one of the non-classical target organs for sex steroids. Presence of androgen and estrogen receptors in the neoplastic and non-neoplastic thyroid glands of manimalian species is well documented. The aim of the present study is to elucidate the changes in serum and thyroidal sex steroids, and their receptors in the thyroid gland of rats from immature to adult age under gonadectomized (GDX) and sex steroids replaced conditions. Normal Wistar male and female rats from immature to adult age (day 21, 30, 45. 60 and 160 post-partum (pp)) were used in the present study. One group (I) of rats was GDX at an early age (day 1 Opp) and the other group (II) at the adult age (day 120 pp). Group I rats were sacrificed at different experimental periods such as 21, 30, 45 and 60 days pp, and group II rats were sacrificed at day 160 pp. Another group of GDX rats from group I and II were replaced with physiological doses of testosterone or estradiol. Serum and thyroidal concentrations of sex steroids were estimated by RIA method and the concentrations of receptors by radioreceptor assay. Gonadectomy significantly decreased serum and thyroidal testosterone and estradiol and concentrations of androgen receptor (AR) and estrogen receptor (ER) in the thyroid. Replacement of sex steroids to GDX rats restored the normal level of sex steroids, AR and ER. Therefore, it is suggested from the present study that (i) sex steroids up-regulate their own receptors in the thyroid, (ii) sex steroids may influence thyroid growth and the proliferation of thyrocytes by modulating their receptor concentrations in the thyroid. (C) 2002 Published by Elsevier Science Inc.