Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.
Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.
复制标题
酿酒酵母 PHO 系统的负调节因子：PHO85 的分离和结构表征。
作者：
Yukifumi Uesono;Kazuma Tanaka;Akio Toh
通过对酿酒酵母PHO85菌株的转化和互补，分离出了其PHO系统的负调控因子PHO85。在1258bp的DNA片段内确定了互补活性，并对该区域进行了测序。在该区域发现的最大的开放阅读框可以编码一个含有302个氨基酸残基的蛋白质。pho85突变是由上述开放阅读框的染色体对应体的破坏引起的。因此，我们得出结论，我们克隆的基因是PHO85。这一结果也表明PHO85不是必需的。Northern分析显示PHO85信息的大小为1.1 kb。PHO80和PHO85两种负调节蛋白的氨基酸序列没有相似性。
One of the negative regulators of the PHO system of Saccharomyces cerevisiae, PHO85, has been isolated by transformation and complementation of a pho85 strain. The complementing activity was delimited within a 1258 bp DNA segment and this region has been sequenced. The largest open reading frame found in this region can encode a protein of 302 amino acid residues. A pho85 mutant resulted from disruption of the chromosomal counterpart of the open reading frame described above. Therefore, we concluded that the gene we have cloned is PHO85. This result also indicates that PHO85 is nonessential. Northern analysis revealed that the size of the PHO85 message is 1.1 kb. No similarity was found between the putative amino acid sequences of two negative regulators, the PHO80 and PHO85 proteins.